Expression and Localization of Sterol 27-hydroxylase (CYP27A1) in Monkey Retina

Overview

Journal Exp Eye Res

Specialty Ophthalmology

Date 2006 Mar 22

PMID 16549062

Citations 29

Authors

Jung Wha Lee

Hirotoshi Fuda

Norman B Javitt

Charles A Strott

Ignacio R Rodriguez

Affiliations

Soon will be listed here.

Abstract

Sterol 27-hydroxylase (CYP27A1) is a mitochondrial P-450 enzyme with broad substrate specificity for C27 sterols including 7-ketocholesterol (7kCh). CYP27A1 is widely expressed in human tissues but has not been previously demonstrated in the retina. In this study, we examined the expression and localization of CYP27A1 in the monkey retina where it localized mainly to the photoreceptor inner segments. CYP27A1 was also observed in Müller cells with faint immuno staining detected in the RPE and choriocapillaris. We also determined that the 27-hydroxylation of 7-ketocholesterol (27OH7kCh) rendered it non-toxic to cultured RPE cells. Moreover, the 27OH7kCh when mixed with 7-ketocholesterol significantly reduced the toxicity of 7-ketocholesterol. These data, when taken in context of the known functions of CYP27A1 imply that expression in the retina serves to modify the biological activity of oxidized sterols that are either transported or generated locally by photo-oxidation.

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