» Articles » PMID: 16542694

Contributions of Autophagic and Apoptotic Mechanisms to CrTX-induced Death of K562 Cells

Overview
Journal Toxicon
Specialty Toxicology
Date 2006 Mar 18
PMID 16542694
Citations 18
Authors
Affiliations
Soon will be listed here.
Abstract

Previous studies reported that the neurotoxin, Crotoxin, isolated from the venom of South American rattlesnake had potent anti-tumor activity. Here, we investigated the involvement of autophagy and apoptosis in the Crotoxin-induced death of chronic myeloid leukemia cell line K562 cells. The neurotoxin dose dependently inhibited the viability of K562 cells. Crotoxin stimulated the autophagic activity as evidenced by the appearance of punctuate monodansylcadaverine (MDC) fluorescence staining in the cytoplasm and increased the formation of autophagosomes. Crotoxin caused the collapse of the mitochondrial membrane potential, release of cytochrome c and activation of caspase-3. Caspase inhibitors attenuated Crotoxin-induced K562 cell death, while blockage of autophagy maturation with 3-methyladenine (3-MA) and NH4Cl potentiated the neurotoxin's cytotoxicity. These results suggest that an apoptotic mechanism contributes to the Crotoxin-induced death of K562 cells, while the activation of autophagy delays neurotoxin-induced apoptosis.

Citing Articles

Venom-derived peptides for breaking through the glass ceiling of drug development.

Freuville L, Matthys C, Quinton L, Gillet J Front Chem. 2024; 12:1465459.

PMID: 39398192 PMC: 11468230. DOI: 10.3389/fchem.2024.1465459.


Crotoxin induces cytotoxic effects in human malignant melanoma cells in both native and detoxified forms.

Almeida T, Giannotti K, Ribeiro Silva L, Marques-Porto R, DeOcesano-Pereira C, Camargo L Front Pharmacol. 2024; 15:1425446.

PMID: 39114354 PMC: 11303296. DOI: 10.3389/fphar.2024.1425446.


Role of Bioactive Compounds in the Regulation of Mitochondrial Dysfunctions in Brain and Age-Related Neurodegenerative Diseases.

Kessas K, Chouari Z, Ghzaiel I, Zarrouk A, Ksila M, Ghrairi T Cells. 2022; 11(2).

PMID: 35053373 PMC: 8773907. DOI: 10.3390/cells11020257.


Novel Approaches of Dysregulating Lysosome Functions in Cancer Cells by Specific Drugs and Its Nanoformulations: A Smart Approach of Modern Therapeutics.

Allemailem K, Almatroudi A, Alrumaihi F, Almatroodi S, Alkurbi M, Basfar G Int J Nanomedicine. 2021; 16:5065-5098.

PMID: 34345172 PMC: 8324981. DOI: 10.2147/IJN.S321343.


Biomedical applications of snake venom: from basic science to autoimmunity and rheumatology.

Canas C, Castano-Valencia S, Castro-Herrera F, Canas F, Tobon G J Transl Autoimmun. 2021; 4:100076.

PMID: 33385156 PMC: 7772571. DOI: 10.1016/j.jtauto.2020.100076.