Nickel-resistant Bacteria from Anthropogenically Nickel-polluted and Naturally Nickel-percolated Ecosystems

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1995 Jun 1

PMID 16535048

Citations 16

Authors

R Stoppel

H G Schlegel

Affiliations

Soon will be listed here.

Abstract

DNA fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in Europe and Zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from New Caledonia by DNA-DNA hybridization. The biotinylated DNA probes were derived from the previously described Alcaligenes eutrophus CH34, Alcaligenes xylosoxidans 31A, Alcaligenes denitrificans 4a-2, and Klebsiella oxytoca CCUG 15788 and four new nickel resistance-determining fragments cloned from strains isolated from soils under nickel-hyperaccumulating trees. Nine probes were hybridized with endonuclease-cleaved plasmid and total DNA samples from 56 nickel-resistant strains. Some of the New Caledonian strains were tentatively identified as Acinetobacter, Pseudomonas mendocina, Comamonas, Hafnia alvei, Burkholderia, Arthrobacter aurescens, and Arthrobacter ramosus strains. The DNA of most strains showed homologies to one or several of the following nickel resistance determinants: the cnr and ncc operons of the strains A. eutrophus CH34 and A. xylosoxidans 31A, respectively, the nre operon of strain 31A, and the nickel resistance determinants of K. oxytoca. On the basis of their hybridization reactions the nickel resistance determinants of the strains could be assigned to four groups: (i) cnr/ncc type, (ii) cnr/ncc/nre type, (iii) K. oxytoca type, and (iv) others. The majority of the strains were assigned to the known groups. Among the strains from Belgium and Zaire, exclusively the cnr/ncc and the cnr/ncc/nre types were found. Among the New Caledonian strains all four types were represented. Homologies to the nre operon were found only in combination with the cnr/ncc operon. The homologies to the cnr/ncc operon were the most abundant and were detected alone or together with homologies to the nre operon. Only the DNA of the strains isolated from soil in Scotland and the United States and that of five of the New Caledonian strains did not show any detectable homologies to any of our probes. The nickel resistance fragment isolated from Burkholderia strain 32W-2 was studied in some detail. This 15-kb BamHI fragment conferred resistance to 1 to 5 mM NiCl(inf2) to Escherichia coli and resistance to up to 25 mM NiCl(inf2) to A. eutrophus. It showed strong homologies to both the cnr/ncc operon and the nre operon and conferred strictly regulated (inducible) nickel resistance to A. eutrophus.

Citing Articles

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The study of Nickel Resistant Bacteria (NiRB) isolated from wastewaters polluted with different industrial sources.

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References

Dong Q, Mergeay M . Czc/cnr efflux: a three-component chemiosmotic antiport pathway with a 12-transmembrane-helix protein. Mol Microbiol. 1994; 14(1):185-7. DOI: 10.1111/j.1365-2958.1994.tb01278.x. View

Hanahan D . Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983; 166(4):557-80. DOI: 10.1016/s0022-2836(83)80284-8. View

Schmidt T, Schlegel H . Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of Alcaligenes xylosoxidans 31A. J Bacteriol. 1994; 176(22):7045-54. PMC: 197079. DOI: 10.1128/jb.176.22.7045-7054.1994. View

Saier Jr M, Tam R, Reizer A, Reizer J . Two novel families of bacterial membrane proteins concerned with nodulation, cell division and transport. Mol Microbiol. 1994; 11(5):841-7. DOI: 10.1111/j.1365-2958.1994.tb00362.x. View

Lonetto M, Brown K, Rudd K, Buttner M . Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions. Proc Natl Acad Sci U S A. 1994; 91(16):7573-7. PMC: 44444. DOI: 10.1073/pnas.91.16.7573. View

Liesegang H, Lemke K, Siddiqui R, Schlegel H . Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34. J Bacteriol. 1993; 175(3):767-78. PMC: 196216. DOI: 10.1128/jb.175.3.767-778.1993. View

WALDE E . [Studies on growth and synthesis of stored substance by Hydrogenomonas]. Arch Mikrobiol. 1962; 43:109-37. View

Diels L, Mergeay M . DNA probe-mediated detection of resistant bacteria from soils highly polluted by heavy metals. Appl Environ Microbiol. 1990; 56(5):1485-91. PMC: 184435. DOI: 10.1128/aem.56.5.1485-1491.1990. View

Schmidt T, Stoppel R, Schlegel H . High-Level Nickel Resistance in Alcaligenes xylosoxydans 31A and Alcaligenes eutrophus KTO2. Appl Environ Microbiol. 1991; 57(11):3301-9. PMC: 183963. DOI: 10.1128/aem.57.11.3301-3309.1991. View

10.

Mergeay M, Houba C, Gerits J . Extrachromosomal inheritance controlling resistance to cadmium, cobalt, copper and zinc ions: evidence from curing in a Pseudomonas [proceedings]. Arch Int Physiol Biochim. 1978; 86(2):440-2. View

11.

Burkardt H, Riess G, Puhler A . Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. J Gen Microbiol. 1979; 114(2):341-8. DOI: 10.1099/00221287-114-2-341. View

12.

Mergeay M . Towards an understanding of the genetics of bacterial metal resistance. Trends Biotechnol. 1991; 9(1):17-24. DOI: 10.1016/0167-7799(91)90007-5. View

13.

Oelmuller U, Schlegel H, Friedrich C . Differential stability of mRNA species of Alcaligenes eutrophus soluble and particulate hydrogenases. J Bacteriol. 1990; 172(12):7057-64. PMC: 210828. DOI: 10.1128/jb.172.12.7057-7064.1990. View

14.

Nies D . Resistance to cadmium, cobalt, zinc, and nickel in microbes. Plasmid. 1992; 27(1):17-28. DOI: 10.1016/0147-619x(92)90003-s. View

15.

Nies A, Nies D, Silver S . Nucleotide sequence and expression of a plasmid-encoded chromate resistance determinant from Alcaligenes eutrophus. J Biol Chem. 1990; 265(10):5648-53. View

16.

Nies A, Nies D, Silver S . Cloning and expression of plasmid genes encoding resistances to chromate and cobalt in Alcaligenes eutrophus. J Bacteriol. 1989; 171(9):5065-70. PMC: 210318. DOI: 10.1128/jb.171.9.5065-5070.1989. View

17.

Siddiqui R, Benthin K, Schlegel H . Cloning of pMOL28-encoded nickel resistance genes and expression of the genes in Alcaligenes eutrophus and Pseudomonas spp. J Bacteriol. 1989; 171(9):5071-8. PMC: 210319. DOI: 10.1128/jb.171.9.5071-5078.1989. View

18.

Mattsby-Baltzer I, Sandin M, Ahlstrom B, Allenmark S, Edebo M, Falsen E . Microbial growth and accumulation in industrial metal-working fluids. Appl Environ Microbiol. 1989; 55(10):2681-9. PMC: 203144. DOI: 10.1128/aem.55.10.2681-2689.1989. View

19.

Nies D, Nies A, Chu L, Silver S . Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus. Proc Natl Acad Sci U S A. 1989; 86(19):7351-5. PMC: 298059. DOI: 10.1073/pnas.86.19.7351. View

20.

Nies D, Mergeay M, Friedrich B, Schlegel H . Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34. J Bacteriol. 1987; 169(10):4865-8. PMC: 213872. DOI: 10.1128/jb.169.10.4865-4868.1987. View