Cloning of Plasmid Genes Encoding Resistance to Cadmium, Zinc, and Cobalt in Alcaligenes Eutrophus CH34

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1987 Oct 1

PMID 2820947

Citations 71

Authors

D Nies

M Mergeay

B Friedrich

H G Schlegel

Affiliations

Soon will be listed here.

Abstract

A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA hybridization, a 9.1-kilobase-pair EcoRI fragment was found to harbor all of these resistance properties and was cloned into the broad-host-range hybrid plasmid pRK290. When transferred to a plasmid-free derivative of CH34, the hybrid plasmid conferred the same degree of resistance as the parent plasmid pMOL30. In two other Alcaligenes strains, the hybrid plasmid was expressed, but to a lower degree than in CH34 derivatives.

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