The Cytochrome Oxidase II Gene in Mitochondria of the Sugar-beet Beta Vulgaris L

Overview

Journal Plant Mol Biol

Date 1991 Sep 1

PMID 1653062

Citations 3

Authors

V Mann

I Ekstein

H Nissen

C Hiser

L McIntosh

J Hirschberg

Affiliations

Soon will be listed here.

Abstract

We have cloned and analyzed the sugar-beet mitochondrial gene for cytochrome oxidase subunit II (coxII). The sugar-beet and its deduced amino acid sequence were compared to its homologous coxII gene sequences from both monocot and dicot plants. It was found to be highly conserved (89-95%) compared to homologue in other plant species. The 780 bp coding sequence of the sugar beet coxII gene is interrupted at position 383 by a 1463 bp intron. This intron contains an additional 107 bp sequence that is not found in any of the plant coxII genes studied thus far. The structure of the intron suggests that a large intron existed in an ancestral coxII gene before monocots and dicots diverged in evolution. Three CGG codons in the sugar-beet coxII coding sequence align with conserved tryptophan residues in the homologous gene of other species, suggesting that RNA editing takes place also in sugar-beet mitochondria. In 13 out of 24 codons of coxII mRNA that were found to be edited in four other plants, the sugar-beet gene already utilizes the edited codons. This phenomenon may indicate that the mitochondrial genome in sugar-beet is phylogenetically more archaic relative to these plants. An additional sequence of 279 bp that is identical to the first exon of coxII was identified in the mtDNA of the sugar-beet. This 'pseudo-gene' is transcribed and its existence in the mitochondrial genome is unexplained.

Citing Articles

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The sugar beet mitochondrial gene for the ATPase alpha-subunit: sequence, transcription and rearrangements in cytoplasmic male-sterile plants.

Senda M, Mikami T, Kinoshita T Curr Genet. 1993; 24(1-2):164-70.

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Conserved sequence blocks 5' to start codons of plant mitochondrial genes.

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References

Covello P, Gray M . RNA editing in plant mitochondria. Nature. 1989; 341(6243):662-6. DOI: 10.1038/341662a0. View

Fox T, Leaver C . The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell. 1981; 26(3 Pt 1):315-23. DOI: 10.1016/0092-8674(81)90200-2. View

Hiesel R, Wissinger B, Brennicke A . Cytochrome oxidase subunit II mRNAs in Oenothera mitochondria are edited at 24 sites. Curr Genet. 1990; 18(4):371-5. DOI: 10.1007/BF00318219. View

Hiesel R, Wissinger B, Schuster W, Brennicke A . RNA editing in plant mitochondria. Science. 1989; 246(4937):1632-4. DOI: 10.1126/science.2480644. View

Pruitt K, Hanson M . Cytochrome oxidase subunit II sequences in Petunia mitochondria: two intron-containing genes and an intron-less pseudogene associated with cytoplasmic male sterility. Curr Genet. 1989; 16(4):281-91. DOI: 10.1007/BF00422115. View

Gray M . Origin and evolution of mitochondrial DNA. Annu Rev Cell Biol. 1989; 5:25-50. DOI: 10.1146/annurev.cb.05.110189.000325. View

Bonen L, Boer P, Gray M . The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. EMBO J. 1984; 3(11):2531-6. PMC: 557724. DOI: 10.1002/j.1460-2075.1984.tb02168.x. View

Mann V, McIntosh L, Theurer C, Hirschberg J . A new cytoplasmic male sterile genotype in the sugar beet Beta vulgaris L.: a molecular analysis. Theor Appl Genet. 2013; 78(2):293-7. DOI: 10.1007/BF00288814. View

Dewey R, Levings 3rd C, Timothy D . Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. 1986; 44(3):439-49. DOI: 10.1016/0092-8674(86)90465-4. View

10.

Kao T, Moon E, Wu R . Cytochrome oxidase subunit II gene of rice has an insertion sequence within the intron. Nucleic Acids Res. 1984; 12(19):7305-15. PMC: 320163. DOI: 10.1093/nar/12.19.7305. View

11.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

12.

Gualberto J, Lamattina L, Bonnard G, Weil J, Grienenberger J . RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature. 1989; 341(6243):660-2. DOI: 10.1038/341660a0. View

13.

Capaldi R, Malatesta F, Darley-Usmar V . Structure of cytochrome c oxidase. Biochim Biophys Acta. 1983; 726(2):135-48. DOI: 10.1016/0304-4173(83)90003-4. View

14.

Schuster W, Wissinger B, Unseld M, Brennicke A . Transcripts of the NADH-dehydrogenase subunit 3 gene are differentially edited in Oenothera mitochondria. EMBO J. 1990; 9(1):263-9. PMC: 551657. DOI: 10.1002/j.1460-2075.1990.tb08104.x. View

15.

Grabau E . Cytochrome oxidase subunit II gene is adjacent to an initiator methionine tRNA gene in soybean mitochondrial DNA. Curr Genet. 1987; 11(4):287-93. DOI: 10.1007/BF00355402. View

16.

Hiesel R, Brennicke A . Cytochrome oxidase subunit II gene in mitochondria of Oenothera has no intron. EMBO J. 1983; 2(12):2173-8. PMC: 555430. DOI: 10.1002/j.1460-2075.1983.tb01719.x. View

17.

Covello P, Gray M . Differences in editing at homologous sites in messenger RNAs from angiosperm mitochondria. Nucleic Acids Res. 1990; 18(17):5189-96. PMC: 332141. DOI: 10.1093/nar/18.17.5189. View

18.

Moon E, Kao T, Wu R . Pea cytochrome oxidase subunit II gene has no intron and generates two mRNA transcripts with different 5'-termini. Nucleic Acids Res. 1985; 13(9):3195-212. PMC: 341229. DOI: 10.1093/nar/13.9.3195. View

19.

YOUNG E, Hanson M, Dierks P . Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit. Nucleic Acids Res. 1986; 14(20):7995-8006. PMC: 311830. DOI: 10.1093/nar/14.20.7995. View

20.

Leaver C, Hack E, Forde B . Protein synthesis by isolated plant mitochondria. Methods Enzymol. 1983; 97:476-84. DOI: 10.1016/0076-6879(83)97156-2. View