The Sugar Beet Mitochondrial Gene for the ATPase Alpha-subunit: Sequence, Transcription and Rearrangements in Cytoplasmic Male-sterile Plants

Overview

Journal Curr Genet

Specialty Genetics

Date 1993 Jul 1

PMID 8358823

Citations 9

Authors

M Senda

T Mikami

T Kinoshita

Affiliations

Soon will be listed here.

Abstract

We have characterized the mitochondrial atpA (the alpha subunit of F1-ATPase) gene from male-fertile cytoplasm (cv TK81-0) of sugar beet. The gene is 1518-bp long and encodes a polypeptide of 506 amino acids. The atpA mRNA sequence is modified by three C-to-U RNA editing events, all of which alter the encoded protein sequences. In order to examine the genome organization of the atpA locus in cytoplasmic male-sterile (CMS) sugar beet, atpA-containing clones were isolated from Owen CMS (TK81-MS) and a different source of CMS [I-12CMS(2)] cytoplasm respectively. The sequences of the atpA coding region from TK81-MS and I-12CMS(2) are identical to each other and to the corresponding TK81-0 sequence. However, the TK81-0 and TK81-MS loci diverge completely 47 bp upstream of the initiation codon, resulting in different 5' transcript termini for the two genes. On the other hand, the point of divergence between the TK81-0 and I-12CMS(2) atpA genes was found to occur after 393 bp 3' to the TAA stop codon. Our results also show the 3'-flanking sequences of I-12CMS(2) atpA to be present elsewhere in the mitochondrial genomes of TK81-0, TK81-MS and I-12CMS(2), suggesting the possible involvement of these repeated DNA elements in the sequence rearrangements.

Citing Articles

Mitochondrial gene variation and phylogenetic relationships in the genus Beta.

Senda M, Onodera Y, Kinoshita T, Mikami T Theor Appl Genet. 2013; 90(7-8):914-9.

PMID: 24173044 DOI: 10.1007/BF00222903.

Mapping of a chloroplast RFLP marker associated with the CMS cytoplasm of sugar beet (Beta vulgaris).

Ran Z, Michaelis G Theor Appl Genet. 2013; 91(6-7):836-40.

PMID: 24169966 DOI: 10.1007/BF00223889.

Large 3' UTR of sugar beet rps3 is truncated in cytoplasmic male-sterile mitochondria.

Matsunaga M, Nagano H, Mikami T, Kubo T Plant Cell Rep. 2010; 30(2):231-8.

PMID: 20711727 DOI: 10.1007/s00299-010-0912-y.

Patterns of partial RNA editing in mitochondrial genes of Beta vulgaris.

Mower J, Palmer J Mol Genet Genomics. 2006; 276(3):285-93.

PMID: 16862402 DOI: 10.1007/s00438-006-0139-3.

The cytoplasmic male-sterile type and normal type mitochondrial genomes of sugar beet share the same complement of genes of known function but differ in the content of expressed ORFs.

Satoh M, Kubo T, Nishizawa S, Estiati A, Itchoda N, Mikami T Mol Genet Genomics. 2004; 272(3):247-56.

PMID: 15340837 DOI: 10.1007/s00438-004-1058-9.

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