Distinct Biochemical Requirements for the Budding, Targeting, and Fusion of ER-derived Transport Vesicles

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1991 Jul 1

PMID 1649197

Citations 122

Authors

M F Rexach

R W Schekman

Affiliations

Soon will be listed here.

Abstract

The transport of pro-alpha-factor from the ER to the Golgi apparatus in gently lysed yeast spheroplasts is mediated by diffusible vesicles. These transport vesicles contain core-glycosylated pro-alpha-factor and are physically separable from donor ER and target Golgi compartments. The formation of diffusible vesicles from the ER requires ATP, Sec12p, Sec23p, and GTP hydrolysis. The vesicles produced are functionally distinct from the ER: they transfer pro-alpha-factor to the Golgi apparatus faster and more efficiently than the ER, they do not require Sec12p or Sec23p to complete transfer, and transfer is resistant to GTP gamma S. Targeting of vesicles to the Golgi apparatus requires Ypt1p and Sec18p. Fusion of vesicles that have targeted requires calcium and ATP.

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References

Novick P, Field C, Schekman R . Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell. 1980; 21(1):205-15. DOI: 10.1016/0092-8674(80)90128-2. View

Novick P, Ferro S, Schekman R . Order of events in the yeast secretory pathway. Cell. 1981; 25(2):461-9. DOI: 10.1016/0092-8674(81)90064-7. View

JAMIESON J, Palade G . Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex. J Cell Biol. 1967; 34(2):577-96. PMC: 2107305. DOI: 10.1083/jcb.34.2.577. View

Segev N, Mulholland J, Botstein D . The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Cell. 1988; 52(6):915-24. DOI: 10.1016/0092-8674(88)90433-3. View

Melancon P, GLICK B, Malhotra V, Weidman P, Serafini T, Gleason M . Involvement of GTP-binding "G" proteins in transport through the Golgi stack. Cell. 1987; 51(6):1053-62. DOI: 10.1016/0092-8674(87)90591-5. View

Schmitt H, Wagner P, Pfaff E, Gallwitz D . The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be involved in microtubule organization. Cell. 1986; 47(3):401-12. DOI: 10.1016/0092-8674(86)90597-0. View

Molenaar C, Prange R, Gallwitz D . A carboxyl-terminal cysteine residue is required for palmitic acid binding and biological activity of the ras-related yeast YPT1 protein. EMBO J. 1988; 7(4):971-6. PMC: 454423. DOI: 10.1002/j.1460-2075.1988.tb02903.x. View

Nakano A, BRADA D, Schekman R . A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. J Cell Biol. 1988; 107(3):851-63. PMC: 2115266. DOI: 10.1083/jcb.107.3.851. View

Ruohola H, Kabcenell A . Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: the acceptor Golgi compartment is defective in the sec23 mutant. J Cell Biol. 1988; 107(4):1465-76. PMC: 2115264. DOI: 10.1083/jcb.107.4.1465. View

10.

Baker D, Hicke L, Rexach M, Schleyer M, Schekman R . Reconstitution of SEC gene product-dependent intercompartmental protein transport. Cell. 1988; 54(3):335-44. DOI: 10.1016/0092-8674(88)90196-1. View

11.

Schmitt H, Puzicha M, Gallwitz D . Study of a temperature-sensitive mutant of the ras-related YPT1 gene product in yeast suggests a role in the regulation of intracellular calcium. Cell. 1988; 53(4):635-47. DOI: 10.1016/0092-8674(88)90579-x. View

12.

Beckers C, Balch W . Calcium and GTP: essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus. J Cell Biol. 1989; 108(4):1245-56. PMC: 2115521. DOI: 10.1083/jcb.108.4.1245. View

13.

Beckers C, Block M, GLICK B, Rothman J, Balch W . Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein. Nature. 1989; 339(6223):397-8. DOI: 10.1038/339397a0. View

14.

Orci L, Malhotra V, Amherdt M, Serafini T, Rothman J . Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack. Cell. 1989; 56(3):357-68. DOI: 10.1016/0092-8674(89)90239-0. View

15.

Balch W . Biochemistry of interorganelle transport. A new frontier in enzymology emerges from versatile in vitro model systems. J Biol Chem. 1989; 264(29):16965-8. View

16.

Franzusoff A, Schekman R . Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation. EMBO J. 1989; 8(9):2695-702. PMC: 401276. DOI: 10.1002/j.1460-2075.1989.tb08410.x. View

17.

Hicke L, Schekman R . Yeast Sec23p acts in the cytoplasm to promote protein transport from the endoplasmic reticulum to the Golgi complex in vivo and in vitro. EMBO J. 1989; 8(6):1677-84. PMC: 401009. DOI: 10.1002/j.1460-2075.1989.tb03559.x. View

18.

Wilson D, Wilcox C, Flynn G, Chen E, Kuang W, Henzel W . A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast. Nature. 1989; 339(6223):355-9. DOI: 10.1038/339355a0. View

19.

Malhotra V, Serafini T, Orci L, Shepherd J, Rothman J . Purification of a novel class of coated vesicles mediating biosynthetic protein transport through the Golgi stack. Cell. 1989; 58(2):329-36. DOI: 10.1016/0092-8674(89)90847-7. View

20.

Block M, GLICK B, Wilcox C, Wieland F, Rothman J . Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport. Proc Natl Acad Sci U S A. 1988; 85(21):7852-6. PMC: 282295. DOI: 10.1073/pnas.85.21.7852. View