GINS, a Central Nexus in the Archaeal DNA Replication Fork

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2006 Feb 18

PMID 16485022

Citations 66

Authors

Nina Marinsek

Elizabeth R Barry

Kira S Makarova

Isabelle Dionne

Eugene V Koonin

Stephen D Bell

Affiliations

Soon will be listed here.

Abstract

In eukaryotes, the GINS complex is essential for DNA replication and has been implicated as having a role at the replication fork. This complex consists of four paralogous GINS subunits, Psf1, Psf2, Psf3 and Sld5. Here, we identify an archaeal GINS homologue as a direct interaction partner of the MCM helicase. The core archaeal GINS complex contains two subunits that are poorly conserved homologues of the eukaryotic GINS subunits, in complex with a protein containing a domain homologous to the DNA-binding domain of bacterial RecJ. Interaction studies show that archaeal GINS interacts directly with the heterodimeric core primase. Our data suggest that GINS is important in coordinating the architecture of the replication fork and provide a mechanism to couple progression of the MCM helicase on the leading strand with priming events on the lagging strand.

Citing Articles

Computational analysis of genes with lethal knockout phenotype and prediction of essential genes in archaea.

Makarova K, Zhang C, Wolf Y, Karamycheva S, Whitaker R, Koonin E mBio. 2024; 15(2):e0309223.

PMID: 38189270 PMC: 10865827. DOI: 10.1128/mbio.03092-23.

Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome.

Oki K, Nagata M, Yamagami T, Numata T, Ishino S, Oyama T Nucleic Acids Res. 2021; 50(7):3601-3615.

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DNA polymerase D temporarily connects primase to the CMG-like helicase before interacting with proliferating cell nuclear antigen.

Oki K, Yamagami T, Nagata M, Mayanagi K, Shirai T, Adachi N Nucleic Acids Res. 2021; 49(8):4599-4612.

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The biology of thermoacidophilic archaea from the order Sulfolobales.

Lewis A, Recalde A, Brasen C, Counts J, Nussbaum P, Bost J FEMS Microbiol Rev. 2021; 45(4).

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-a model archaeon for studying DNA replication and repair.

Perez-Arnaiz P, Dattani A, Smith V, Allers T Open Biol. 2020; 10(12):200293.

PMID: 33259746 PMC: 7776575. DOI: 10.1098/rsob.200293.

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