Increased Response to High KCl-induced Elevation in the Intracellular-Ca(2+) Concentration in Differentiated NG108-15 Cell and the Inhibitory Effect of the L-type Ca(2+) Channel Blocker, Calciseptine

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2006 Feb 14

PMID 16474994

Citations 9

Authors

Takashi Imanishi

Kayoko Matsushima

Akinori Kawaguchi

Tetsuyuki Wada

Shigeru Yoshida

Seiji Ichida

Affiliations

Soon will be listed here.

Abstract

Characteristics of the increasing effect for the concentration of intracellular calcium ions ([Ca(2+)](i)) by high-KCl application were investigated in the neuroblastomaxglioma hybrid NG108-15 cell line (NG108-15 cells). The present study confirmed that the increasing effect of [Ca(2+)](i) by high-KCl application in single NG108-15 cells, differentiated with dibutyryl cAMP (Bt(2)cAMP), was significantly enhanced, compared to undifferentiated cells. The following observations were made at first: (1) The response to high-KCl application, in both undifferentiated and differentiated cells, was significantly inhibited by calciseptine (CaS), an L-type Ca(2+) channel blocker, but not by N-, P- and R-type Ca(2+) channel blockers. The IC(50) values for CaS in both undifferentiated and differentiated cell was almost identical. (2) The inhibitory effect of CaS was irreversible. (3) The increasing effect for [Ca(2+)](i) by high-KCl application was completely dependent on the presence of extracellular calcium ions. (4) The increased [Ca(2+)](i) by high-KCl application under a plateau concentration was quickly decreased to basal levels when the high-KCl solution was exchanged for a high-KCl solution containing EGTA (without CaCl(2)). Together, these results suggest that the enhancement of the response effect of [Ca(2+)](i) by high-KCl application in differentiated single NG108-15 cells was mainly due to the quantitative increase of L-type voltage-sensitive calcium channels (VSCCs), which were irreversibly inhibited by CaS.

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References

Berridge M . Neuronal calcium signaling. Neuron. 1998; 21(1):13-26. DOI: 10.1016/s0896-6273(00)80510-3. View

Bader C, Bertrand D, Dupin E, Kato A . Development of electrical membrane properties in cultured avian neural crest. Nature. 1983; 305(5937):808-10. DOI: 10.1038/305808a0. View

Catterall W . Structure and regulation of voltage-gated Ca2+ channels. Annu Rev Cell Dev Biol. 2000; 16:521-55. DOI: 10.1146/annurev.cellbio.16.1.521. View

Nirenberg M, Wilson S, Higashida H, Rotter A, Krueger K, Busis N . Modulation of synapse formation by cyclic adenosine monophosphate. Science. 1983; 222(4625):794-9. DOI: 10.1126/science.6314503. View

Tojima T, Kobayashi S, Ito E . Dual role of cyclic AMP-dependent protein kinase in neuritogenesis and synaptogenesis during neuronal differentiation. J Neurosci Res. 2003; 74(6):829-37. DOI: 10.1002/jnr.10754. View

Kim H, Song M, Kim E, Ryu S, Suh P . Dexamethasone differentiates NG108-15 cells through cyclooxygenase 1 induction. Exp Mol Med. 2003; 35(3):203-10. DOI: 10.1038/emm.2003.28. View

Tojima T, Ito E . A cyclic AMP-regulated negative feedforward system for neuritogenesis revealed in a neuroblastomaxglioma hybrid cell line. Neuroscience. 2001; 104(2):583-91. DOI: 10.1016/s0306-4522(01)00061-6. View

Ichida S, Matsuda N, NAKAZAKI S, Kishino H, Wada T, Akimoto T . Characteristics of 45Ca uptake stimulated by high KCl of differentiated and undifferentiated NG108-15 and PC12h cells. Neurochem Res. 1993; 18(5):625-32. DOI: 10.1007/BF00966941. View

Kasai H, Neher E . Dihydropyridine-sensitive and omega-conotoxin-sensitive calcium channels in a mammalian neuroblastoma-glioma cell line. J Physiol. 1992; 448:161-88. PMC: 1176193. DOI: 10.1113/jphysiol.1992.sp019035. View

10.

McGee R, Simpson P, Christian C, Mata M, Nelson P, Nirenberg M . Regulation of acetylcholine release from neuroblastoma x glioma hybrid cells. Proc Natl Acad Sci U S A. 1978; 75(3):1314-8. PMC: 411461. DOI: 10.1073/pnas.75.3.1314. View

11.

Grynkiewicz G, Poenie M, Tsien R . A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem. 1985; 260(6):3440-50. View

12.

Hirsh J, Quandt F . Down-regulation of Na channel expression by A23187 in N1E-115 neuroblastoma cells. Brain Res. 1996; 706(2):343-6. DOI: 10.1016/0006-8993(95)01340-7. View

13.

Monjaraz E, Navarrete A, Lopez-Santiago L, Vega A, Cota G . L-type calcium channel activity regulates sodium channel levels in rat pituitary GH3 cells. J Physiol. 2000; 523 Pt 1:45-55. PMC: 2269790. DOI: 10.1111/j.1469-7793.2000.00045.x. View

14.

Daniels M, Hamprecht B . The ultrastructure of neuroblastoma glioma somatic cell hybrids. Expression of neuronal characteristics stimulated by dibutyryl adenosine 3',5' cyclic monophosphate. J Cell Biol. 1974; 63(2 Pt 1):691-9. PMC: 2110941. DOI: 10.1083/jcb.63.2.691. View

15.

De Weille J, Schweitz H, Maes P, Tartar A, Lazdunski M . Calciseptine, a peptide isolated from black mamba venom, is a specific blocker of the L-type calcium channel. Proc Natl Acad Sci U S A. 1991; 88(6):2437-40. PMC: 51247. DOI: 10.1073/pnas.88.6.2437. View

16.

Hamprecht B . Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int Rev Cytol. 1977; 49:99-170. DOI: 10.1016/s0074-7696(08)61948-8. View

17.

Ichida S, Wada T, NAKAZAKI S, Matsuda N, Kishino H, Akimoto T . Specific bindings of [3H](+)PN200-110 and [125I]omega-conotoxin to crude membranes from differentiated NG108-15 cells. Neurochem Res. 1993; 18(5):633-8. DOI: 10.1007/BF00966942. View

18.

Nowycky M, Fox A, Tsien R . Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985; 316(6027):440-3. DOI: 10.1038/316440a0. View

19.

Seeds N, GILMAN A, Amano T, Nirenberg M . Regulation of axon formation by clonal lines of a neural tumor. Proc Natl Acad Sci U S A. 1970; 66(1):160-7. PMC: 286102. DOI: 10.1073/pnas.66.1.160. View

20.

Wyatt C, Page K, Berrow N, Brice N, Dolphin A . The effect of overexpression of auxiliary Ca2+ channel subunits on native Ca2+ channel currents in undifferentiated mammalian NG108-15 cells. J Physiol. 1998; 510 ( Pt 2):347-60. PMC: 2231049. DOI: 10.1111/j.1469-7793.1998.347bk.x. View