Dissimilar Kinetic Behavior of Electrically Manipulated Single- and Double-stranded DNA Tethered to a Gold Surface

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2006 Feb 14

PMID 16473909

Citations 9

Authors

Ulrich Rant

Kenji Arinaga

Marc Tornow

Yong Woon Kim

Roland R Netz

Shozo Fujita

Naoki Yokoyama

Gerhard Abstreiter

Affiliations

Soon will be listed here.

Abstract

We report on the electrical manipulation of single- and double-stranded oligodeoxynucleotides that are end tethered to gold surfaces in electrolyte solution. The response to alternating repulsive and attractive electric surface fields is studied by time-resolved fluorescence measurements, revealing markedly distinct dynamics for the flexible single-stranded and stiff double-stranded DNA, respectively. Hydrodynamic simulations rationalize this finding and disclose two different kinetic mechanisms: stiff polymers undergo rotation around the anchoring pivot point; flexible polymers, on the other hand, are pulled onto the attracting surface segment by segment.

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References

Liu Q, Wang L, Frutos A, Condon A, Corn R, Smith L . DNA computing on surfaces. Nature. 2000; 403(6766):175-9. DOI: 10.1038/35003155. View

Boon E, Salas J, Barton J . An electrical probe of protein-DNA interactions on DNA-modified surfaces. Nat Biotechnol. 2002; 20(3):282-6. DOI: 10.1038/nbt0302-282. View

Heller M . DNA microarray technology: devices, systems, and applications. Annu Rev Biomed Eng. 2002; 4:129-53. DOI: 10.1146/annurev.bioeng.4.020702.153438. View

Bustamante C, Bryant Z, Smith S . Ten years of tension: single-molecule DNA mechanics. Nature. 2003; 421(6921):423-7. DOI: 10.1038/nature01405. View

Rant U, Arinaga K, Fujita S, Yokoyama N, Abstreiter G, Tornow M . Structural properties of oligonucleotide monolayers on gold surfaces probed by fluorescence investigations. Langmuir. 2004; 20(23):10086-92. DOI: 10.1021/la0492963. View