Electrical Potential-assisted DNA-RNA Hybridization for Rapid MicroRNA Extraction

Overview

Journal Anal Bioanal Chem

Specialty Chemistry

Date 2022 Mar 1

PMID 35229173

Authors

Xiaoli Zhao

Yong Li

Ritong Sun

Yaofang Fan

Xiaofeng Mu

Ye Wang

Chao Shi

Cuiping Ma

Affiliations

Soon will be listed here.

Abstract

Analysis of microRNAs (miRNAs) is important in cancer diagnostics and therapy. Conventional methods used to extract miRNA for analysis are generally time-consuming. A novel approach for rapid and sensitive extraction of miRNAs is urgently need for clinical applications. Herein, a novel strategy based on electrical potential-assisted DNA-RNA hybridization was designed for miRNA extraction. The entire extraction process was accomplished in approximately 3 min, which is much shorter than the commercial adsorption column method, at more than 60 min, or the TRIzol method, at more than 90 min. Additionally, the method offered the advantages of simplicity and specificity during the extraction process by electrical potential-assisted hybridization of single-stranded DNA and RNA. Taking let-7a as an example, satisfactory results were achieved for miRNA extraction in serum, demonstrating the applicability in miRNA nucleic acid amplification.

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