Receptor for Advanced Glycation End-products is a Marker of Type I Cell Injury in Acute Lung Injury

Overview

Journal Am J Respir Crit Care Med

Specialty Critical Care

Date 2006 Feb 4

PMID 16456142

Citations 234

Authors

Tokujiro Uchida

Madoka Shirasawa

Lorraine B Ware

Katsuo Kojima

Yutaka Hata

Koshi Makita

Gabe Mednick

Zachary A Matthay

Michael A Matthay

Affiliations

Soon will be listed here.

Abstract

Rationale: Receptor for advanced glycation end-products (RAGE) is one of the alveolar type I cell-associated proteins in the lung.

Objectives: To test the hypothesis that RAGE is a marker of alveolar epithelial type I cell injury.

Methods: Rats were instilled intratracheally with 10 mg/kg lipopolysaccharide or hydrochloric acid. RAGE levels were measured in the bronchoalveolar lavage (BAL) and serum in the rats and in the pulmonary edema fluid and plasma from patients with acute lung injury (ALI; n = 22) and hydrostatic pulmonary edema (n = 11).

Main Results: In the rat lung injury studies, RAGE was released into the BAL and serum as a single soluble isoform sized approximately 48 kD. The elevated levels of RAGE in the BAL correlated well with the severity of experimentally induced lung injury. In the human studies, the RAGE level in the pulmonary edema fluid was significantly higher than the plasma level (p < 0.0001). The median edema fluid/plasma ratio of RAGE levels was 105 (interquartile range, 55-243). The RAGE levels in the pulmonary edema fluid from patients with ALI were higher than the levels from patients with hydrostatic pulmonary edema (p < 0.05), and the plasma RAGE level in patients with ALI were significantly higher than the healthy volunteers (p < 0.001) or patients with hydrostatic pulmonary edema (p < 0.05).

Conclusion: RAGE is a marker of type I alveolar epithelial cell injury based on experimental studies in rats and in patients with ALI.

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