5' Analysis of the Soybean Leghaemoglobin Lbc(3) Gene: Regulatory Elements Required for Promoter Activity and Organ Specificity

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1987 Dec 1

PMID 16453808

Citations 72

Authors

J Stougaard

N N Sandal

A Gron

A Kuhle

K A Marcker

Affiliations

Soon will be listed here.

Abstract

The soybean leghaemoglobin lbc(3) gene promoter was analysed in transgenic Lotus corniculatus plants. Hybrid-promoter constructions and 5' deletions were studied using chimeric genes composed of the various promoters, the chloramphenicol acetyltransferase (CAT) coding sequence and the lbc(3) 3' flanking region. A 5' Bal31 deletion series mapped a strong positive regulatory element between -1100 and -950. A weaker element located between -230 and -170 defined the minimum 5' region required for detectable promoter activity. Reactivation of inactive promoters with deletion endpoints between -230 and the transcription initiation site was obtained employing the constitutive cauliflower mosaic virus (CaMV) 35S enhancer. The position of cis regulatory element(s) required for nodule-specific expression was defined to 37 bp between -139 and -102. This region contains sequences conserved in other leghaemoglobin and nodulin genes. No indispensable control elements were found on the lbc(3) 3' flanking region.

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References

Fuchsman W, Appleby C . Separation and determination of the relative concentrations of the homogeneous components of soybean leghemoglobin by isoelectric focusing. Biochim Biophys Acta. 1979; 579(2):314-24. DOI: 10.1016/0005-2795(79)90059-x. View

Chen Z, Schuler M, Beachy R . Functional analysis of regulatory elements in a plant embryo-specific gene. Proc Natl Acad Sci U S A. 1986; 83(22):8560-4. PMC: 386970. DOI: 10.1073/pnas.83.22.8560. View

Jensen E, Marcker K, Villadsen I . Heme regulates the expression in Saccharomyces cerevisiae of chimaeric genes containing 5'-flanking soybean leghemoglobin sequences. EMBO J. 1986; 5(5):843-7. PMC: 1166872. DOI: 10.1002/j.1460-2075.1986.tb04293.x. View

Hattori M, Sakaki Y . Dideoxy sequencing method using denatured plasmid templates. Anal Biochem. 1986; 152(2):232-8. DOI: 10.1016/0003-2697(86)90403-3. View

Peters N, Frost J, Long S . A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes. Science. 1986; 233(4767):977-80. DOI: 10.1126/science.3738520. View

Sandal N, Bojsen K, Marcker K . A small family of nodule specific genes from soybean. Nucleic Acids Res. 1987; 15(4):1507-19. PMC: 340564. DOI: 10.1093/nar/15.4.1507. View

Timko M, Kausch A, Castresana C, FASSLER J, Herrera-Estrella L, Van den Broeck G . Light regulation of plant gene expression by an upstream enhancer-like element. Nature. 1985; 318(6046):579-82. DOI: 10.1038/318579a0. View

Wiborg O, Jensen E, Paludan K, Marcker K . The nucleotide sequences of two leghemoglobin genes from soybean. Nucleic Acids Res. 1982; 10(11):3487-94. PMC: 320725. DOI: 10.1093/nar/10.11.3487. View

Brisson N, Verma D . Soybean leghemoglobin gene family: normal, pseudo, and truncated genes. Proc Natl Acad Sci U S A. 1982; 79(13):4055-9. PMC: 346575. DOI: 10.1073/pnas.79.13.4055. View

10.

Gielen J, De Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M . The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5. EMBO J. 1984; 3(4):835-46. PMC: 557436. DOI: 10.1002/j.1460-2075.1984.tb01894.x. View

11.

Franck A, Guilley H, Jonard G, Richards K, Hirth L . Nucleotide sequence of cauliflower mosaic virus DNA. Cell. 1980; 21(1):285-94. DOI: 10.1016/0092-8674(80)90136-1. View

12.

Ellis J, Llewellyn D, Dennis E, Peacock W . Maize Adh-1 promoter sequences control anaerobic regulation: addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco. EMBO J. 1987; 6(1):11-6. PMC: 553349. DOI: 10.1002/j.1460-2075.1987.tb04711.x. View

13.

Marcker A, Lund M, Jensen E, Marcker K . Transcription of the soybean leghemoglobin genes during nodule development. EMBO J. 1984; 3(8):1691-5. PMC: 557583. DOI: 10.1002/j.1460-2075.1984.tb02033.x. View

14.

Stougaard J, Petersen T, Marcker K . Expression of a complete soybean leghemoglobin gene in root nodules of transgenic Lotus corniculatus. Proc Natl Acad Sci U S A. 1987; 84(16):5754-7. PMC: 298941. DOI: 10.1073/pnas.84.16.5754. View

15.

Jensen E, Paludan K, Wiborg O, Garrett R, Jorgensen P, Marcker K . The primary structures of two leghemoglobin genes from soybean. Nucleic Acids Res. 1982; 10(2):689-701. PMC: 326171. DOI: 10.1093/nar/10.2.689. View

16.

Dierks P, Ooyen A, Cochran M, Dobkin C, Reiser J, Weissmann C . Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells. Cell. 1983; 32(3):695-706. DOI: 10.1016/0092-8674(83)90055-7. View

17.

Legocki R, Verma D . Identification of "nodule-specific" host proteins (nodoulins) involved in the development of rhizobium-legume symbiosis. Cell. 1980; 20(1):153-63. DOI: 10.1016/0092-8674(80)90243-3. View

18.

Herrera-Estrella L, Block M, Messens E, Hernalsteens J, Montagu M, Schell J . Chimeric genes as dominant selectable markers in plant cells. EMBO J. 1983; 2(6):987-95. PMC: 555219. DOI: 10.1002/j.1460-2075.1983.tb01532.x. View

19.

Simpson J, Timko M, Cashmore A, Schell J, Montagu M, Herrera-Estrella L . Light-inducible and tissue-specific expression of a chimaeric gene under control of the 5'-flanking sequence of a pea chlorophyll a/b-binding protein gene. EMBO J. 1985; 4(11):2723-9. PMC: 554570. DOI: 10.1002/j.1460-2075.1985.tb03995.x. View

20.

Fluhr R, Kuhlemeier C, Nagy F, Chua N . Organ-specific and light-induced expression of plant genes. Science. 1986; 232(4754):1106-12. DOI: 10.1126/science.232.4754.1106. View