Host-specific Regulation of Nodulation Genes in Rhizobium is Mediated by a Plant-signal, Interacting with the NodD Gene Product

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1987 Apr 1

PMID 16453758

Citations 76

Authors

B Horvath

C W Bachem

J Schell

A Kondorosi

Affiliations

Soon will be listed here.

Abstract

We have identified a nodD gene from the wide host-range Rhizobium strain MPIK3030 (termed nodD1) which is essential for nodulation on Macroptilium atropurpureum (siratro). Experiments with nodA-lacZ gene fusions demonstrate that the MPIK3030 nodD1 regulates expression of the nodABC genes. Additionally, we used nodC-lacZ fusions of Rhizobium meliloti to show that the MPIK3030 nodD1 gene induces expression of these fusions by interacting with plant factors from siratro and from the non-host Medicago sativa (alfalfa). The R. meliloti nodD genes, however, only interact with alfalfa exudate. In line with these results, no complementation of MPIK3030 nodD1 mutants could be obtained on siratro with the R. meliloti nodD genes, while the MPIK3030 nodD1 can complement nodD mutants of R. meliloti on alfalfa. Furthermore, R. meliloti transconjugants harbouring the MPIK3030 nodD1 efficiently nodulate the illegitimate host siratro. When compared with other nodD sequences, the amino acid sequence of the MPIK3030 nodD1 shows a conserved aminoterminus, whereas the carboxy-terminus of the putative gene product diverges considerably. Studies on a chimeric MPIK3030/R. meliloti nodD gene indicates that the carboxy-terminal region is responsible for the interaction with plant factor(s) and may have evolved in different rhizobia specifically to interact with plant-host factors.

Citing Articles

The Rhizobium tropici CIAT 899 NodD2 protein promotes symbiosis and extends rhizobial nodulation range by constitutive nodulation factor synthesis.

Ayala-Garcia P, Jimenez-Guerrero I, Jacott C, Lopez-Baena F, Ollero F, Del Cerro P J Exp Bot. 2022; 73(19):6931-6941.

PMID: 35901852 PMC: 9629785. DOI: 10.1093/jxb/erac325.

Rhizobium leguminosarum bv. trifolii NodD2 Enhances Competitive Nodule Colonization in the Clover-Rhizobium Symbiosis.

Ferguson S, Major A, Sullivan J, Bourke S, Kelly S, Perry B Appl Environ Microbiol. 2020; 86(18).

PMID: 32651206 PMC: 7480365. DOI: 10.1128/AEM.01268-20.

Phylogeny and distribution of Bradyrhizobium symbionts nodulating cowpea (Vigna unguiculata L. Walp) and their association with the physicochemical properties of acidic African soils.

Puozaa D, Jaiswal S, Dakora F Syst Appl Microbiol. 2019; 42(3):403-414.

PMID: 30803810 PMC: 6542415. DOI: 10.1016/j.syapm.2019.02.004.

Cross-exchange of B-vitamins underpins a mutualistic interaction between Ostreococcus tauri and Dinoroseobacter shibae.

Cooper M, Kazamia E, Helliwell K, Kudahl U, Sayer A, Wheeler G ISME J. 2018; 13(2):334-345.

PMID: 30228381 PMC: 6331578. DOI: 10.1038/s41396-018-0274-y.

Genetic and Molecular Mechanisms Underlying Symbiotic Specificity in Legume-Rhizobium Interactions.

Wang Q, Liu J, Zhu H Front Plant Sci. 2018; 9:313.

PMID: 29593768 PMC: 5854654. DOI: 10.3389/fpls.2018.00313.

References

Gottfert M, Horvath B, Kondorosi E, Putnoky P, Rodriguez-Quinones F, Kondorosi A . At least two nodD genes are necessary for efficient nodulation of alfalfa by Rhizobium meliloti. J Mol Biol. 1986; 191(3):411-20. DOI: 10.1016/0022-2836(86)90136-1. View

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

Broughton W, Wong C, Lewin A, Samrey U, Myint H, Meyer H . Identification of Rhizobium plasmid sequences involved in recognition of Psophocarpus, Vigna, and other legumes. J Cell Biol. 1986; 102(4):1173-82. PMC: 2114177. DOI: 10.1083/jcb.102.4.1173. View

Scott K . Conserved nodulation genes from the non-legume symbiont Bradyrhizobium sp. (Parasponia). Nucleic Acids Res. 1986; 14(7):2905-19. PMC: 339711. DOI: 10.1093/nar/14.7.2905. View

Egelhoff T, Fisher R, Jacobs T, Mulligan J, Long S . Nucleotide sequence of Rhizobium meliloti 1021 nodulation genes: nodD is read divergently from nodABC. DNA. 1985; 4(3):241-8. DOI: 10.1089/dna.1985.4.241. View

Beringer J . R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974; 84(1):188-98. DOI: 10.1099/00221287-84-1-188. View

Orosz L, Svab Z, Kondorosi A, Sik T . Genetic studies on rhizobiophage 16-3. I. Genes and functions on the chromosome. Mol Gen Genet. 1973; 125(4):341-50. View

Boyer H . A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969; 41(3):459-72. DOI: 10.1016/0022-2836(69)90288-5. View

HANAU R, Koduri R, Ho N, Brenchley J . Nucleotide sequence of the control regions for the glnA and glnL genes of Salmonella typhimurium. J Bacteriol. 1983; 155(1):82-9. PMC: 217655. DOI: 10.1128/jb.155.1.82-89.1983. View

10.

Rostas K, Kondorosi E, Horvath B, Simoncsits A, Kondorosi A . Conservation of extended promoter regions of nodulation genes in Rhizobium. Proc Natl Acad Sci U S A. 1986; 83(6):1757-61. PMC: 323163. DOI: 10.1073/pnas.83.6.1757. View

11.

Norrander J, Kempe T, Messing J . Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983; 26(1):101-6. DOI: 10.1016/0378-1119(83)90040-9. View

12.

Castilho B, Olfson P, Casadaban M . Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. J Bacteriol. 1984; 158(2):488-95. PMC: 215454. DOI: 10.1128/jb.158.2.488-495.1984. View

13.

Torok I, Kondorosi E, Stepkowski T, Posfai J, Kondorosi A . Nucleotide sequence of Rhizobium meliloti nodulation genes. Nucleic Acids Res. 1984; 12(24):9509-24. PMC: 320477. DOI: 10.1093/nar/12.24.9509. View

14.

Stragier P, PATTE J . Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. III. Nucleotide sequence and regulation of the lysR gene. J Mol Biol. 1983; 168(2):333-50. DOI: 10.1016/s0022-2836(83)80022-9. View

15.

Rossen L, Johnston A, Downie J . DNA sequence of the Rhizobium leguminosarum nodulation genes nodAB and C required for root hair curling. Nucleic Acids Res. 1984; 12(24):9497-508. PMC: 320476. DOI: 10.1093/nar/12.24.9497. View

16.

Banfalvi Z, Sakanyan V, Koncz C, Kiss A, Dusha I, Kondorosi A . Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti. Mol Gen Genet. 1981; 184(2):318-25. DOI: 10.1007/BF00272925. View

17.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

18.

Casadaban M . Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976; 104(3):541-55. DOI: 10.1016/0022-2836(76)90119-4. View

19.

Casadaban M . Fusion of the Escherichia coli lac genes to the ara promoter: a general technique using bacteriophage Mu-1 insertions. Proc Natl Acad Sci U S A. 1975; 72(3):809-13. PMC: 432409. DOI: 10.1073/pnas.72.3.809. View

20.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View