Conservation of Extended Promoter Regions of Nodulation Genes in Rhizobium

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Mar 1

PMID 16593668

Citations 80

Authors

K Rostas

E Kondorosi

B Horvath

A Simoncsits

A Kondorosi

Affiliations

Soon will be listed here.

Abstract

A 47-base-pair (bp) conserved sequence in the 5'-flanking regions of three transcriptional units coding for nodulation functions (nodABC, nodEFG, and nodH) has been identified in Rhizobium meliloti strain 41. The conserved region contains subsequences of 7 bp, 5 bp, and 25 bp. The conserved 25-bp sequence was synthesized and used as a hybridization probe; three additional copies of the sequence were identified in R. meliloti 41; all three were localized in the 135-kb nod/nif region of the symbiotic megaplasmid. Nucleotide sequence analysis of the six regions revealed that all contained the 47-bp conserved sequence but, with one exception, adjacent DNA regions did not have long conserved DNA stretches. The position of the 47-bp region was about 200-240 bp upstream of the translational start codons of the three nod genes. This conserved sequence is present in several other Rhizobium species and located adjacent to nod genes. We have demonstrated the involvement of this sequence in the expression of nodulation functions, which suggests that these extended promoter regions may have a role in the coordinated regulation of nodulation genes.

Citing Articles

Nod factor perception: an integrative view of molecular communication during legume symbiosis.

Ghantasala S, Roy Choudhury S Plant Mol Biol. 2022; 110(6):485-509.

PMID: 36040570 DOI: 10.1007/s11103-022-01307-3.

Symbiosis islands of Loteae-nodulating comprise three radiating lineages with concordant gene complements and nodulation host-range groupings.

Perry B, Sullivan J, Colombi E, Murphy R, Ramsay J, Ronson C Microb Genom. 2020; 6(9).

PMID: 32845829 PMC: 7643969. DOI: 10.1099/mgen.0.000426.

Rhizobium leguminosarum bv. trifolii NodD2 Enhances Competitive Nodule Colonization in the Clover-Rhizobium Symbiosis.

Ferguson S, Major A, Sullivan J, Bourke S, Kelly S, Perry B Appl Environ Microbiol. 2020; 86(18).

PMID: 32651206 PMC: 7480365. DOI: 10.1128/AEM.01268-20.

Genetic and Molecular Mechanisms Underlying Symbiotic Specificity in Legume-Rhizobium Interactions.

Wang Q, Liu J, Zhu H Front Plant Sci. 2018; 9:313.

PMID: 29593768 PMC: 5854654. DOI: 10.3389/fpls.2018.00313.

Transcriptomic Studies of the Effect of nod Gene-Inducing Molecules in Rhizobia: Different Weapons, One Purpose.

Jimenez-Guerrero I, Acosta-Jurado S, Del Cerro P, Navarro-Gomez P, Lopez-Baena F, Ollero F Genes (Basel). 2017; 9(1).

PMID: 29267254 PMC: 5793154. DOI: 10.3390/genes9010001.

References

Moore J, Dixon B . Metastasis of a transplantable mammary tumour in rats treated with cyclophosphamide and/or irradiation. Br J Cancer. 1977; 36(2):221-6. PMC: 2025474. DOI: 10.1038/bjc.1977.181. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Jacobs T, Egelhoff T, Long S . Physical and genetic map of a Rhizobium meliloti nodulation gene region and nucleotide sequence of nodC. J Bacteriol. 1985; 162(2):469-76. PMC: 218872. DOI: 10.1128/jb.162.2.469-476.1985. View

Banfalvi Z, Kondorosi E, Kondorosi A . Rhizobium meliloti carries two megaplasmids. Plasmid. 1985; 13(2):129-38. DOI: 10.1016/0147-619x(85)90065-4. View

Egelhoff T, Fisher R, Jacobs T, Mulligan J, Long S . Nucleotide sequence of Rhizobium meliloti 1021 nodulation genes: nodD is read divergently from nodABC. DNA. 1985; 4(3):241-8. DOI: 10.1089/dna.1985.4.241. View

Boyer H . A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969; 41(3):459-72. DOI: 10.1016/0022-2836(69)90288-5. View

Berg D, Weiss A, Crossland L . Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol. 1980; 142(2):439-46. PMC: 293997. DOI: 10.1128/jb.142.2.439-446.1980. View

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

Better M, Lewis B, Corbin D, Ditta G, Helinski D . Structural relationships among Rhizobium meliloti symbiotic promoters. Cell. 1983; 35(2 Pt 1):479-85. DOI: 10.1016/0092-8674(83)90181-2. View

10.

Torok I, Kondorosi E, Stepkowski T, Posfai J, Kondorosi A . Nucleotide sequence of Rhizobium meliloti nodulation genes. Nucleic Acids Res. 1984; 12(24):9509-24. PMC: 320477. DOI: 10.1093/nar/12.24.9509. View

11.

Drummond M, Clements J, Merrick M, Dixon R . Positive control and autogenous regulation of the nifLA promoter in Klebsiella pneumoniae. Nature. 1983; 301(5898):302-7. DOI: 10.1038/301302a0. View

12.

Rossen L, Johnston A, Downie J . DNA sequence of the Rhizobium leguminosarum nodulation genes nodAB and C required for root hair curling. Nucleic Acids Res. 1984; 12(24):9497-508. PMC: 320476. DOI: 10.1093/nar/12.24.9497. View

13.

Banfalvi Z, Sakanyan V, Koncz C, Kiss A, Dusha I, Kondorosi A . Location of nodulation and nitrogen fixation genes on a high molecular weight plasmid of R. meliloti. Mol Gen Genet. 1981; 184(2):318-25. DOI: 10.1007/BF00272925. View

14.

Rosenberg C, Boistard P, Denarie J . Genes controlling early and late functions in symbiosis are located on a megaplasmid in Rhizobium meliloti. Mol Gen Genet. 1981; 184(2):326-33. DOI: 10.1007/BF00272926. View

15.

Miyada C, Soberon X, Itakura K, Wilcox G . The use of synthetic oligodeoxyribonucleotides to produce specific deletions in the araBAD promoter of Escherichia coli B/r. Gene. 1982; 17(2):167-77. DOI: 10.1016/0378-1119(82)90070-1. View

16.

Downie J, Ma Q, Knight C, Hombrecher G, Johnston A . Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene. EMBO J. 1983; 2(6):947-52. PMC: 555213. DOI: 10.1002/j.1460-2075.1983.tb01526.x. View

17.

Corbin D, Barran L, Ditta G . Organization and expression of Rhizobium meliloti nitrogen fixation genes. Proc Natl Acad Sci U S A. 1983; 80(10):3005-9. PMC: 393962. DOI: 10.1073/pnas.80.10.3005. View