Mapping the Structure and Function of the E1 and E2 Glycoproteins in Alphaviruses

Overview

Journal Structure

Publisher Cell Press

Specialties Biochemistry
Biotechnology
Cell Biology
Molecular Biology

Date 2006 Jan 13

PMID 16407066

Citations 101

Authors

Suchetana Mukhopadhyay

Wei Zhang

Stefan Gabler

Paul R Chipman

Ellen G Strauss

James H Strauss

Timothy S Baker

Richard J Kuhn

Michael G Rossmann

Affiliations

Soon will be listed here.

Abstract

The 9 A resolution cryo-electron microscopy map of Sindbis virus presented here provides structural information on the polypeptide topology of the E2 protein, on the interactions between the E1 and E2 glycoproteins in the formation of a heterodimer, on the difference in conformation of the two types of trimeric spikes, on the interaction between the transmembrane helices of the E1 and E2 proteins, and on the conformational changes that occur when fusing with a host cell. The positions of various markers on the E2 protein established the approximate topology of the E2 structure. The largest conformational differences between the icosahedral surface spikes at icosahedral 3-fold and quasi-3-fold positions are associated with the monomers closest to the 5-fold axes. The long E2 monomers, containing the cell receptor recognition motif at their extremities, are shown to rotate by about 180 degrees and to move away from the center of the spikes during fusion.

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