Replicon Typing of Virulence Plasmids of Enterotoxigenic Escherichia Coli Isolates from Cattle

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1992 Aug 1

PMID 1639505

Citations 9

Authors

J G Mainil

F Bex

P Dreze

A KAECKENBEECK

M Couturier

Affiliations

Soon will be listed here.

Abstract

Plasmid DNA hybridization with probes for virulence factors used for basic replicons of plasmids was used to identify the virulence plasmids of a collection of enterotoxigenic Escherichia coli isolates from cattle. The virulence probes were derived from the genes coding for the heat-stable enterotoxin STaP and for the F5 (K99) and F41 fimbrial adhesins. The replicon probes were derived from 16 different basic replicons of plasmids (probes repFIA, repFIB, repFIC, repFIIA, repI1, repHI1, repHI2, repL/M, repN, repP, repQ, repT, repU, repW, repX, and repY). The virulence genes coding for the STaP enterotoxin and for the F5 adhesin were located on a single plasmid band in each isolate. The sizes of most of these virulence plasmids were from 65 to 95 MDa. The F41 probe failed to hybridize with any plasmid band. The virulence plasmids had multireplicon types typical of plasmids of the IncF groups. The most common basic replicon association was the triple RepFIA-RepFIB-RepFIC family association.

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References

So M, Boyer H, Betlach M, Falkow S . Molecular cloning of an Escherichia coli plasmid determinant than encodes for the production of heat-stable enterotoxin. J Bacteriol. 1976; 128(1):463-72. PMC: 232874. DOI: 10.1128/jb.128.1.463-472.1976. View

So M, Heffron F, McCarthy B . The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1. Nature. 1979; 277(5696):453-6. DOI: 10.1038/277453a0. View

Moon H, Whipp S, Skartvedt S . Etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and K99 antigen production by Escherichia cola. Am J Vet Res. 1976; 37(9):1025-9. View

Falkow S, WILLIAMS Jr L, Seaman S, Rollins L . Increased survival in calves of Escherichia coli K-12 carrying an Ent plasmid. Infect Immun. 1976; 13(3):1005-7. PMC: 420709. DOI: 10.1128/iai.13.3.1005-1007.1976. View

Casey T, Moon H . Genetic characterization and virulence of enterotoxigenic Escherichia coli mutants which have lost virulence genes in vivo. Infect Immun. 1990; 58(12):4156-8. PMC: 313791. DOI: 10.1128/iai.58.12.4156-4158.1990. View

Holland R . Some infectious causes of diarrhea in young farm animals. Clin Microbiol Rev. 1990; 3(4):345-75. PMC: 358168. DOI: 10.1128/CMR.3.4.345. View

Saadi S, Maas W, Hill D, Bergquist P . Nucleotide sequence analysis of RepFIC, a basic replicon present in IncFI plasmids P307 and F, and its relation to the RepA replicon of IncFII plasmids. J Bacteriol. 1987; 169(5):1836-46. PMC: 212033. DOI: 10.1128/jb.169.5.1836-1846.1987. View

Bergquist P, Saadi S, Maas W . Distribution of basic replicons having homology with RepFIA, RepFIB, and RepFIC among IncF group plasmids. Plasmid. 1986; 15(1):19-34. DOI: 10.1016/0147-619x(86)90011-9. View

Moseley S, Dougan G, Schneider R, Moon H . Cloning of chromosomal DNA encoding the F41 adhesin of enterotoxigenic Escherichia coli and genetic homology between adhesins F41 and K88. J Bacteriol. 1986; 167(3):799-804. PMC: 215944. DOI: 10.1128/jb.167.3.799-804.1986. View

10.

Mainil J, Moseley S, Schneider R, Sutch K, Casey T, Moon H . Hybridization of bovine Escherichia coli isolates with gene probes for four enterotoxins (STaP, STaH, STb, LT) and one adhesion factor (K99). Am J Vet Res. 1986; 47(5):1145-8. View

11.

Wong K, Tan L, Saddler J . Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications. Microbiol Rev. 1988; 52(3):305-17. PMC: 373146. DOI: 10.1128/mr.52.3.305-317.1988. View

12.

Shimizu M, Sakano T, Satoh S . Linkage of K99 production and STa activity in a plasmid of an Escherichia coli porcine isolate. Microbiol Immunol. 1988; 32(6):635-9. DOI: 10.1111/j.1348-0421.1988.tb01424.x. View

13.

Broes A, Fairbrother J, Mainil J, Harel J, Lariviere S . Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli serotype O8:KX105 and O8:K"2829" strains isolated from piglets with diarrhea. J Clin Microbiol. 1988; 26(11):2402-9. PMC: 266901. DOI: 10.1128/jcm.26.11.2402-2409.1988. View

14.

Anderson D, Moseley S . Escherichia coli F41 adhesin: genetic organization, nucleotide sequence, and homology with the K88 determinant. J Bacteriol. 1988; 170(10):4890-6. PMC: 211534. DOI: 10.1128/jb.170.10.4890-4896.1988. View

15.

Silva R, Saadi S, Maas W . A basic replicon of virulence-associated plasmids of Shigella spp. and enteroinvasive Escherichia coli is homologous with a basic replicon in plasmids of IncF groups. Infect Immun. 1988; 56(4):836-42. PMC: 259378. DOI: 10.1128/iai.56.4.836-842.1988. View

16.

Pohl P, Lintermans P, Bex F, Desmyter A, Dreze P, Fonteyne P . [Phenotypic and genotypic properties of 4 virulence plasmids of Salmonella typhimurium]. Ann Inst Pasteur Microbiol. 1987; 138(5):523-8. View

17.

Murray B, Tsao J . Comparison of CFA/I:ST plasmids of enterotoxigenic Escherichia coli. J Infect Dis. 1987; 156(2):398-401. DOI: 10.1093/infdis/156.2.398. View

18.

Echeverria P, Seriwatana J, Taylor D, Changchawalit S, SMYTH C, Twohig J . Plasmids coding for colonization factor antigens I and II, heat-labile enterotoxin, and heat-stable enterotoxin A2 in Escherichia coli. Infect Immun. 1986; 51(2):626-30. PMC: 262392. DOI: 10.1128/iai.51.2.626-630.1986. View

19.

Bertin A . F41 antigen as a virulence factor in the infant mouse model of Escherichia coli diarrhoea. J Gen Microbiol. 1985; 131(11):3037-45. DOI: 10.1099/00221287-131-11-3037. View

20.

Maas R, Silva R, Gomes T, Trabulsi L, Maas W . Detection of genes for heat-stable enterotoxin I in Escherichia coli strains isolated in Brazil. Infect Immun. 1985; 49(1):46-51. PMC: 262056. DOI: 10.1128/iai.49.1.46-51.1985. View