The Quorum-sensing Negative Regulator RsaL of Pseudomonas Aeruginosa Binds to the LasI Promoter

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2005 Dec 31

PMID 16385073

Citations 47

Authors

Giordano Rampioni

Iris Bertani

Elisabetta Zennaro

Fabio Polticelli

Vittorio Venturi

Livia Leoni

Affiliations

Soon will be listed here.

Abstract

A mutation in the rsaL gene of Pseudomonas aeruginosa produces dramatically higher amounts of N-acyl homoserine lactone with respect to the wild type, highlighting the key role of this negative regulator in controlling quorum sensing (QS) in this opportunistic pathogen. The DNA binding site of the RsaL protein on the rsaL-lasI bidirectional promoter partially overlaps the binding site of the LasR protein, consistent with the hypothesis that RsaL and LasR could be in binding competition on this promoter. This is the first direct demonstration that RsaL acts as a QS negative regulator by binding to the lasI promoter.

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