Plasmid Transfer into the Homoacetogen Acetobacterium Woodii by Electroporation and Conjugation

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1994 Mar 1

PMID 16349209

Citations 9

Authors

M Stratz

U Sauer

A Kuhn

P Durre

Affiliations

Soon will be listed here.

Abstract

Shuttle vectors (pMS3 and pMS4) which replicated in Escherichia coli and in gram-positive Acetobacterium woodii were constructed by ligating the replication origin of plasmid pAMbeta1 with the E. coli cloning vector pUC19 and the tetM gene of streptococcal transposon Tn916. Electrotransformation of A. woodii was achieved at frequencies of 4.5 x 10 transformants per mug of plasmid DNA. For conjugal plasmid transfer, the mobilizable shuttle vector pKV12 was constructed by cloning the tetM gene into pAT187. Mating of E. coli containing pKV12 with A. woodii resulted in transfer frequencies of 3 x 10 to 7 x 10 per donor or recipient.

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