Isozyme Variation Among 40 Frankia Strains

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1987 Jul 1

PMID 16347388

Citations 6

Authors

M Gardes

J Bousquet

M Lalonde

Affiliations

Soon will be listed here.

Abstract

Forty Frankia strains belonging to the Alnus and Elaeagnus host specificity groups and isolated from various plant species from different geographical areas were characterized by the electrophoretic separation of isozymes of eight enzymes. All the enzyme systems that were investigated showed large variation. Diaphorases and esterases gave multiple band patterns and confirmed the identification of specific Frankia strains. Less variability was observed with enzymes such as phosphoglucose isomerase, leucine aminopeptidase, and malate dehydrogenase, which allowed for the delineation of larger groups of Frankia strains. Cluster analysis, based on the pair-wise similarity coefficients calculated between strains, delineated three large, dissimilar groups of Frankia strains, although each of these groups contained a large amount of heterogeneity. However, numerous Frankia strains, mainly from the Alnus host specificity group, demonstrated a perfect homology for all the enzymes tested.

Citing Articles

Diversity and specificity of Frankia strains in nodules of sympatric Myrica gale, Alnus incana, and Shepherdia canadensis determined by rrs gene polymorphism.

Huguet V, Batzli J, Zimpfer J, Normand P, Dawson J, Fernandez M Appl Environ Microbiol. 2001; 67(5):2116-22.

PMID: 11319089 PMC: 92844. DOI: 10.1128/AEM.67.5.2116-2122.2001.

Enzyme polymorphism, prodigiosin production, and plasmid fingerprints in clinical and naturally occurring isolates of Serratia marcescens.

Gargallo-Viola D J Clin Microbiol. 1989; 27(5):860-8.

PMID: 2663918 PMC: 267444. DOI: 10.1128/jcm.27.5.860-868.1989.

Phylogeny of nitrogenase sequences in Frankia and other nitrogen-fixing microorganisms.

Normand P, Bousquet J J Mol Evol. 1989; 29(5):436-47.

PMID: 2515293 DOI: 10.1007/BF02602914.

Phylogenetic relationships among Frankia genomic species determined by use of amplified 16S rDNA sequences.

Nazaret S, Cournoyer B, Normand P, Simonet P J Bacteriol. 1991; 173(13):4072-8.

PMID: 2061287 PMC: 208055. DOI: 10.1128/jb.173.13.4072-4078.1991.

High-molecular-mass multicatalytic proteinase complexes produced by the nitrogen-fixing actinomycete Frankia strain BR.

Benoist P, Muller A, Diem H, Schwencke J J Bacteriol. 1992; 174(5):1495-504.

PMID: 1537794 PMC: 206544. DOI: 10.1128/jb.174.5.1495-1504.1992.

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