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Two Improved Methods for Obtaining Axenic Cultures of Cyanobacteria

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 1979 Nov 1
PMID 16345455
Citations 19
Authors
T Vaara
M Vaara
S Niemela
Affiliations
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Abstract

Scoring the agar plate before incubation under unidirectional light led to a rapid separation of gliding filamentous cyanobacteria from their contaminating bacteria. Twenty strains were purified by the method. Additionally, 13 axenic cyanobacterial strains were isolated from pour plates made after treatment of cyanobacterial cultures in tryptone-yeast extract-glucose broth with cycloserine in darkness to select for obligate photoautotrophs.

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