A Cell-autonomous Requirement for the Cell Cycle Regulatory Protein, Rb, in Neuronal Migration

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2005 Nov 26

PMID 16308563

Citations 38

Authors

Kerry L Ferguson

Kelly A McClellan

Jacqueline L Vanderluit

William C McIntosh

Carol Schuurmans

Franck Polleux

Ruth S Slack

Affiliations

Soon will be listed here.

Abstract

Precise cell cycle regulation is critical for nervous system development. To assess the role of the cell cycle regulator, retinoblastoma (Rb) protein, in forebrain development, we studied mice with telencephalon-specific Rb deletions. We examined the role of Rb in neuronal specification and migration of diverse neuronal populations. Although layer specification occurred at the appropriate time in Rb mutants, migration of early-born cortical neurons was perturbed. Consistent with defects in radial migration, neuronal cell death in Rb mutants specifically affected Cajal-Retzius neurons. In the ventral telencephalon, although calbindin- and Lhx6-expressing cortical neurons were generated at embryonic day 12.5, their tangential migration into the neocortex was dramatically and specifically reduced in the mutant marginal zone. Cell transplantation assays revealed that defects in tangential migration arose owing to a cell-autonomous loss of Rb in migrating interneurons and not because of a defective cortical environment. These results revealed a cell-autonomous role for Rb in regulating the tangential migration of cortical interneurons. Taken together, we reveal a novel requirement for the cell cycle protein, Rb, in the regulation of neuronal migration.

Citing Articles

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Core cell cycle machinery is crucially involved in both life and death of post-mitotic neurons.

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