Kinetics of Active Sucrose Transport in Saccharomyces Cerevisiae

Overview

Journal J Biosci Bioeng

Specialties Biochemistry
Biomedical Engineering
Microbiology

Date 2005 Oct 20

PMID 16232731

Citations 17

Authors

B U Stambuk

A S Batista

P S de Araujo

Affiliations

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Abstract

The kinetic analysis of active sucrose-H+ uptake by Saccharomyces cerevisiae revealed the presence of two transport systems with high and low affinity for sucrose. The MAL2T permease has a low affinity (K(m) = 120 +/-20 mM) for sucrose, while the alpha-glucoside transporter encoded by the AGT1 gene is a high affinity sucrose-H+ symporter (K(m) = 7.9+/-0.8 mM) that increases the specific growth rate of cells growing on sucrose.

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