Sequences Intervening Between the Core Packaging Determinants Are Dispensable for Maintaining the Packaging Potential and Propagation of Feline Immunodeficiency Virus Transfer Vector RNAs

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2005 Oct 18

PMID 16227303

Citations 20

Authors

Farah Mustafa

Akela Ghazawi

Preethi Jayanth

Pretty Susan Phillip

Jahabar Ali

Tahir A Rizvi

Affiliations

Soon will be listed here.

Abstract

The packaging determinants of feline immunodeficiency virus (FIV) consist of two discontinuous core regions, extending from R to approximately 150 bp of the 5' untranslated region and the first approximately 100 bp of gag. However, the role of sequences intervening between the core regions in packaging has not been clear. A mutational analysis was conducted to determine whether the intervening sequences played a role in FIV RNA packaging, using an in vivo packaging assay complemented with semiquantitative reverse transcriptase PCR. Our analyses reveal that the intervening sequences are dispensable not only for vector RNA packaging but also for propagation, confirming the discontinuous nature of the FIV packaging signal.

Citing Articles

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Identification of a putative Gag binding site critical for feline immunodeficiency virus genomic RNA packaging.

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Role of Purine-Rich Regions in Mason-Pfizer Monkey Virus (MPMV) Genomic RNA Packaging and Propagation.

Ali L, Pitchai F, Vivet-Boudou V, Chameettachal A, Jabeen A, Pillai V Front Microbiol. 2020; 11:595410.

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Purification and Functional Characterization of a Biologically Active Full-Length Feline Immunodeficiency Virus (FIV) Pr50.

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