Evaluation of Immunoglobulin G Subclass Antibodies Against Recombinant Fasciola Gigantica Cathepsin L1 in an Enzyme-linked Immunosorbent Assay for Serodiagnosis of Human Fasciolosis

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2005 Oct 8

PMID 16210476

Citations 14

Authors

Chaisiri Wongkham

Chairat Tantrawatpan

Pewpan M Intapan

Wanchai Maleewong

Sopit Wongkham

Kunio Nakashima

Affiliations

Soon will be listed here.

Abstract

A cystatin capture enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola gigantica cathepsin L1 antigen was developed to detect specific immunoglobulin G (IgG) subclass antibodies (IgG1, IgG2, IgG3, and IgG4) and was evaluated for its diagnostic potential for human fasciolosis. In an analysis of the sera of 13 patients infected with F. gigantica, 209 patients with other parasitic infections, 32 cholangiocarcinoma patients, and 42 healthy controls, the IgG4-ELISA gave the highest diagnostic values. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method based on the detection of IgG4 antibody were 100%, 99.3%, 99.3%, 86.7%, and 100%, respectively. The results revealed that restricting the ELISA to the detection of specific IgG4 antibody enhanced the specificity and accuracy for the serodiagnosis of human fasciolosis.

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