A High-resolution Physical Map of Equine Homologs of HSA19 Shows Divergent Evolution Compared with Other Mammals

Overview

Journal Mamm Genome

Specialty Genetics

Date 2005 Sep 24

PMID 16180145

Citations 11

Authors

Candice Brinkmeyer-Langford

Terje Raudsepp

Eun-Joon Lee

Glenda Goh

Alejandro A Schaffer

Richa Agarwala

Michelle L Wagner

Teruaki Tozaki

Loren C Skow

James E Womack

James R Mickelson

Bhanu P Chowdhary

Affiliations

Soon will be listed here.

Abstract

A high-resolution (1 marker/700 kb) physically ordered radiation hybrid (RH) and comparative map of 122 loci on equine homologs of human Chromosome 19 (HSA19) shows a variant evolution of these segments in equids/Perissodactyls compared with other mammals. The segments include parts of both the long and the short arm of horse Chromosome 7 (ECA7), the proximal part of ECA21, and the entire short arm of ECA10. The map includes 93 new markers, of which 89 (64 gene-specific and 25 microsatellite) were genotyped on a 5000-rad horse x hamster RH panel, and 4 were mapped exclusively by FISH. The orientation and alignment of the map was strengthened by 21 new FISH localizations, of which 15 represent genes. The approximately sevenfold-improved map resolution attained in this study will prove extremely useful for candidate gene discovery in the targeted equine chromosomal regions. The highlight of the comparative map is the fine definition of homology between the four equine chromosomal segments and corresponding HSA19 regions specified by physical coordinates (bp) in the human genome sequence. Of particular interest are the regions on ECA7 and ECA21 that correspond to the short arm of HSA19-a genomic rearrangement discovered to date only in equids/Perissodactyls as evidenced through comparative Zoo-FISH analysis of the evolution of ancestral HSA19 segments in eight mammalian orders involving about 50 species.

Citing Articles

Subchromosomal karyotype evolution in Equidae.

Musilova P, Kubickova S, Vahala J, Rubes J Chromosome Res. 2013; 21(2):175-87.

PMID: 23532666 DOI: 10.1007/s10577-013-9346-z.

Karyotypic relationships in Asiatic asses (kulan and kiang) as defined using horse chromosome arm-specific and region-specific probes.

Musilova P, Kubickova S, Horin P, Vodicka R, Rubes J Chromosome Res. 2009; 17(6):783-90.

PMID: 19731053 DOI: 10.1007/s10577-009-9069-3.

A high resolution RH map of the bovine major histocompatibility complex.

Brinkmeyer-Langford C, Childers C, Fritz K, Gustafson-Seabury A, Cothran M, Raudsepp T BMC Genomics. 2009; 10:182.

PMID: 19393056 PMC: 2682492. DOI: 10.1186/1471-2164-10-182.

A 4,103 marker integrated physical and comparative map of the horse genome.

Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner M, Goh G, Seabury C Cytogenet Genome Res. 2008; 122(1):28-36.

PMID: 18931483 PMC: 2587302. DOI: 10.1159/000151313.

Glycogen synthase (GYS1) mutation causes a novel skeletal muscle glycogenosis.

McCue M, Valberg S, Miller M, Wade C, DiMauro S, Akman H Genomics. 2008; 91(5):458-66.

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