Cooperative Interaction of Angiopoietin-like Proteins 1 and 2 in Zebrafish Vascular Development

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2005 Sep 22

PMID 16174743

Citations 43

Authors

Yoshiaki Kubota

Yuichi Oike

Shinya Satoh

Yoko Tabata

Yuichi Niikura

Tohru Morisada

Masaki Akao

Takashi Urano

Yasuhiro Ito

Takeshi Miyamoto

Norihiro Nagai

Gou Young Koh

Sumiko Watanabe

Toshio Suda

Affiliations

Soon will be listed here.

Abstract

Angiopoietin-like protein (Angptl) 1 and Angptl2, which are considered orphan ligands, are highly homologous, particularly in the fibrinogen-like domain containing the putative receptor binding site. This similarity suggests potentially cooperative functions between the two proteins. In this report, the function of Angptl1 and Angptl2 is analyzed by using morpholino antisense technology in zebrafish. Knockdown of both Angptl1 and Angptl2 produced severe vascular defects due to increased apoptosis of endothelial cells at the sprouting stage. In vitro studies showed that Angptl1 and Angptl2 have antiapoptotic activities through the phosphatidylinositol 3-kinase/Akt pathway, and coinjection of constitutively active Akt/protein kinase B mRNA rescued impaired vascular development seen in double knockdown embryos. These results provide a physiological demonstration of the cooperative interaction of Angptl1 and Angptl2 in endothelial cells through phosphatidylinositol 3-kinase/Akt mediated antiapoptotic activities.

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