Aurora A Phosphorylation of TACC3/maskin is Required for Centrosome-dependent Microtubule Assembly in Mitosis

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2005 Sep 21

PMID 16172205

Citations 142

Authors

Kazuhisa Kinoshita

Tim L Noetzel

Laurence Pelletier

Karl Mechtler

David N Drechsel

Anne Schwager

Mike Lee

Jordan W Raff

Anthony A Hyman

Affiliations

Soon will be listed here.

Abstract

Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.

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