Constraining Ribosomal RNA Conformational Space

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2005 Sep 13

PMID 16155182

Citations 5

Authors

Paola Favaretto

Arjun Bhutkar

Temple F Smith

Affiliations

Soon will be listed here.

Abstract

Despite the potential for many possible secondary-structure conformations, the native sequence of ribosomal RNA (rRNA) is able to find the correct and universally conserved core fold. This study reports a computational analysis investigating two mechanisms that appear to constrain rRNA secondary-structure conformational space: ribosomal proteins and rRNA sequence composition. The analysis was carried out by using rRNA-ribosomal protein interaction data for the Escherichia coli 16S rRNA and free energy minimization software for secondary-structure prediction. The results indicate that selection pressures on rRNA sequence composition and ribosomal protein-rRNA interaction play a key role in constraining the rRNA secondary structure to a single stable form.

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