Occurrence of PS86/pEF47-related Plasmids in Gram-positive Cocci

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2005 Aug 11

PMID 16091849

Citations 1

Authors

Adriana Sprincova

Viliam Stovcik

Peter Javorsky

Peter Pristas

Affiliations

Soon will be listed here.

Abstract

A small, low-copy-number cryptic 5.5-kb plasmid, designated pEF47, was isolated from a rumen bacterium Enterococcus faecalis 47 and partially characterized. Comparisons by restriction and partial sequence analysis of pEF47 demonstrated high homology to pS86 plasmid, recently isolated from a human clinical strain E. faecalis S-86. PCR followed by sequence analysis and Southern hybridization showed that pS86/pEF47-related replicons are regularly encountered in plasmids from gram-positive cocci.

Citing Articles

Development of experimental genetic tools for Campylobacter fetus.

Kienesberger S, Gorkiewicz G, Joainig M, Scheicher S, Leitner E, Zechner E Appl Environ Microbiol. 2007; 73(14):4619-30.

PMID: 17513579 PMC: 1932827. DOI: 10.1128/AEM.02407-06.

References

Murray B, Singh K, Ross R, Heath J, Dunny G, Weinstock G . Generation of restriction map of Enterococcus faecalis OG1 and investigation of growth requirements and regions encoding biosynthetic function. J Bacteriol. 1993; 175(16):5216-23. PMC: 204989. DOI: 10.1128/jb.175.16.5216-5223.1993. View

Paulsen I, Banerjei L, Myers G, Nelson K, Seshadri R, Read T . Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 2003; 299(5615):2071-4. DOI: 10.1126/science.1080613. View

Dunny G, Leonard B, Hedberg P . Pheromone-inducible conjugation in Enterococcus faecalis: interbacterial and host-parasite chemical communication. J Bacteriol. 1995; 177(4):871-6. PMC: 176677. DOI: 10.1128/jb.177.4.871-876.1995. View

Singh K, Murray B . Revised estimates of enterococcal chromosomal sizes. DNA Cell Biol. 1994; 13(11):1145-6. DOI: 10.1089/dna.1994.13.1145. View

Clewell D, Yagi Y, Dunny G, Schultz S . Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J Bacteriol. 1974; 117(1):283-9. PMC: 246555. DOI: 10.1128/jb.117.1.283-289.1974. View

Weisburg W, Barns S, Pelletier D, Lane D . 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 1991; 173(2):697-703. PMC: 207061. DOI: 10.1128/jb.173.2.697-703.1991. View

Francia M, Clewell D . Amplification of the tetracycline resistance determinant of pAMalpha1 in Enterococcus faecalis requires a site-specific recombination event involving relaxase. J Bacteriol. 2002; 184(18):5187-93. PMC: 135321. DOI: 10.1128/JB.184.18.5187-5193.2002. View

Franz C, Holzapfel W, Stiles M . Enterococci at the crossroads of food safety?. Int J Food Microbiol. 1999; 47(1-2):1-24. DOI: 10.1016/s0168-1605(99)00007-0. View

Anderson D, McKay L . Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983; 46(3):549-52. PMC: 239314. DOI: 10.1128/aem.46.3.549-552.1983. View

10.

Wyckoff H, Barnes M, Gillies K, Sandine W . Characterization and sequence analysis of a stable cryptic plasmid from Enterococcus faecium 226 and development of a stable cloning vector. Appl Environ Microbiol. 1996; 62(4):1481-6. PMC: 167923. DOI: 10.1128/aem.62.4.1481-1486.1996. View

11.

Miranda A, Singh K, Murray B . Determination of the chromosomal size of three different strains of Enterococcus faecalis and one strain of Enterococcus faecium. DNA Cell Biol. 1992; 11(4):331-5. DOI: 10.1089/dna.1992.11.331. View

12.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

13.

Pospiech A, Neumann B . A versatile quick-prep of genomic DNA from gram-positive bacteria. Trends Genet. 1995; 11(6):217-8. DOI: 10.1016/s0168-9525(00)89052-6. View

14.

Kalkus J, Dorrie C, Fischer D, Reh M, Schlegel H . The giant linear plasmid pHG207 from Rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini. J Gen Microbiol. 1993; 139(9):2055-65. DOI: 10.1099/00221287-139-9-2055. View

15.

Tailor S, Bailey E, Rybak M . Enterococcus, an emerging pathogen. Ann Pharmacother. 1993; 27(10):1231-42. DOI: 10.1177/106002809302701014. View

16.

Whitford M, McPherson M, Forster R, Teather R . Identification of bacteriocin-like inhibitors from rumen Streptococcus spp. and isolation and characterization of bovicin 255. Appl Environ Microbiol. 2001; 67(2):569-74. PMC: 92622. DOI: 10.1128/AEM.67.2.569-574.2001. View

17.

Quednau M, Ahrne S, Molin G . Genomic relationships between Enterococcus faecium strains from different sources and with different antibiotic resistance profiles evaluated by restriction endonuclease analysis of total chromosomal DNA using EcoRI and PvuII. Appl Environ Microbiol. 1999; 65(4):1777-80. PMC: 91251. DOI: 10.1128/AEM.65.4.1777-1780.1999. View

18.

Styriak I, Laukova A, Fallgren C, Wadstrom T . Binding of selected extracellular matrix proteins to enterococci and Streptococcus bovis of animal origin. Curr Microbiol. 1999; 39(6):327-0335. DOI: 10.1007/s002849900467. View

19.

Martinez-Bueno M, Valdivia E, Galvez A, Maqueda M . pS86, a new theta-replicating plasmid from Enterococcus faecalis. Curr Microbiol. 2000; 41(4):257-61. DOI: 10.1007/s002840010130. View