Degradation of Crude Oil by an Arctic Microbial Consortium

Overview

Journal Extremophiles

Publisher Springer

Date 2005 Jul 7

PMID 15999222

Citations 28

Authors

Uta Deppe

Hans-Hermann Richnow

Walter Michaelis

Garabed Antranikian

Affiliations

Soon will be listed here.

Abstract

The ability of a psychrotolerant microbial consortium to degrade crude oil at low temperatures was investigated. The enriched arctic microbial community was also tested for its ability to utilize various hydrocarbons, such as long-chain alkanes (n-C24 to n-C34), pristane, (methyl-)naphthalenes, and xylenes, as sole carbon and energy sources. Except for o-xylene and methylnaphthalenes, all tested compounds were metabolized under conditions that are typical for contaminated marine liquid sites, namely at pH 6-9 and at 4-27 degrees C. By applying molecular biological techniques (16S rDNA sequencing, DGGE) nine strains could be identified in the consortium. Five of these strains could be isolated in pure cultures. The involved strains were closely related to the following genera: Pseudoalteromonas (two species), Pseudomonas (two species), Shewanella (two species), Marinobacter (one species), Psychrobacter (one species), and Agreia (one species). Interestingly, the five isolated strains in different combinations were unable to degrade crude oil or its components significantly, indicating the importance of the four unculturable microorganisms in the degradation of single or of complex mixtures of hydrocarbons. The obtained mixed culture showed obvious advantages including stability of the consortium, wide range adaptability for crude oil degradation, and strong degradation ability of crude oil.

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References

Van Hamme J, Odumeru J, Ward O . Community dynamics of a mixed-bacterial culture growing on petroleum hydrocarbons in batch culture. Can J Microbiol. 2000; 46(5):441-50. View

Whyte L, Hawari J, Zhou E, Bourbonniere L, Inniss W, Greer C . Biodegradation of variable-chain-length alkanes at low temperatures by a psychrotrophic Rhodococcus sp. Appl Environ Microbiol. 1998; 64(7):2578-84. PMC: 106429. DOI: 10.1128/AEM.64.7.2578-2584.1998. View

Polz M, Cavanaugh C . Bias in template-to-product ratios in multitemplate PCR. Appl Environ Microbiol. 1998; 64(10):3724-30. PMC: 106531. DOI: 10.1128/AEM.64.10.3724-3730.1998. View

Friedrich M, Grosser R, Kern E, Inskeep W, Ward D . Effect of model sorptive phases on phenanthrene biodegradation: molecular analysis of enrichments and isolates suggests selection based on bioavailability. Appl Environ Microbiol. 2000; 66(7):2703-10. PMC: 92063. DOI: 10.1128/AEM.66.7.2703-2710.2000. View

Whyte L, Slagman S, Pietrantonio F, Bourbonniere L, Koval S, Lawrence J . Physiological adaptations involved in alkane assimilation at a low temperature by Rhodococcus sp. strain Q15. Appl Environ Microbiol. 1999; 65(7):2961-8. PMC: 91443. DOI: 10.1128/AEM.65.7.2961-2968.1999. View

Morita R . Psychrophilic bacteria. Bacteriol Rev. 1975; 39(2):144-67. PMC: 413900. DOI: 10.1128/br.39.2.144-167.1975. View

Kasai Y, Kishira H, Syutsubo K, Harayama S . Molecular detection of marine bacterial populations on beaches contaminated by the Nakhodka tanker oil-spill accident. Environ Microbiol. 2001; 3(4):246-55. DOI: 10.1046/j.1462-2920.2001.00185.x. View

Leahy J, Colwell R . Microbial degradation of hydrocarbons in the environment. Microbiol Rev. 1990; 54(3):305-15. PMC: 372779. DOI: 10.1128/mr.54.3.305-315.1990. View

Pelz O, Tesar M, Wittich R, Moore E, Timmis K, Abraham W . Towards elucidation of microbial community metabolic pathways: unravelling the network of carbon sharing in a pollutant-degrading bacterial consortium by immunocapture and isotopic ratio mass spectrometry. Environ Microbiol. 2001; 1(2):167-74. DOI: 10.1046/j.1462-2920.1999.00023.x. View

10.

Harayama S, Kishira H, Kasai Y, Shutsubo K . Petroleum biodegradation in marine environments. J Mol Microbiol Biotechnol. 2000; 1(1):63-70. View

11.

Bartlett D, Silverman M . Nucleotide sequence of IS492, a novel insertion sequence causing variation in extracellular polysaccharide production in the marine bacterium Pseudomonas atlantica. J Bacteriol. 1989; 171(3):1763-6. PMC: 209814. DOI: 10.1128/jb.171.3.1763-1766.1989. View

12.

Syutsubo K, Kishira H, Harayama S . Development of specific oligonucleotide probes for the identification and in situ detection of hydrocarbon-degrading Alcanivorax strains. Environ Microbiol. 2001; 3(6):371-9. DOI: 10.1046/j.1462-2920.2001.00204.x. View

13.

Westlake D, Jobson A, Phillippe R, Cook F . Biodegradability and crude oil composition. Can J Microbiol. 1974; 20(7):915-28. DOI: 10.1139/m74-141. View

14.

Jobson A, Cook F, Westlake D . Microbial utilization of crude oil. Appl Microbiol. 1972; 23(6):1082-9. PMC: 380511. DOI: 10.1128/am.23.6.1082-1089.1972. View

15.

Margesin R, Schinner F . Efficiency of indigenous and inoculated cold-adapted soil microorganisms for biodegradation of diesel oil in alpine soils. Appl Environ Microbiol. 1997; 63(7):2660-4. PMC: 1389197. DOI: 10.1128/aem.63.7.2660-2664.1997. View

16.

Raghukumar C, Vipparty V, David J, Chandramohan D . Degradation of crude oil by marine cyanobacteria. Appl Microbiol Biotechnol. 2002; 57(3):433-6. DOI: 10.1007/s002530100784. View

17.

Sahm K, Knoblauch C, Amann R . Phylogenetic affiliation and quantification of psychrophilic sulfate-reducing isolates in marine Arctic sediments. Appl Environ Microbiol. 1999; 65(9):3976-81. PMC: 99729. DOI: 10.1128/AEM.65.9.3976-3981.1999. View

18.

Annweiler E, Richnow H, Antranikian G, Hebenbrock S, Garms C, Franke S . Naphthalene degradation and incorporation of naphthalene-derived carbon into biomass by the thermophile Bacillus thermoleovorans. Appl Environ Microbiol. 2000; 66(2):518-23. PMC: 91857. DOI: 10.1128/AEM.66.2.518-523.2000. View

19.

Gross M, Logan B . Influence of different chemical treatments on transport of Alcaligenes paradoxus in porous media. Appl Environ Microbiol. 1995; 61(5):1750-6. PMC: 167437. DOI: 10.1128/aem.61.5.1750-1756.1995. View

20.

Gauthier M, LAFAY B, Christen R, Fernandez L, Acquaviva M, Bonin P . Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol. 1992; 42(4):568-76. DOI: 10.1099/00207713-42-4-568. View