Phylogenetic Affiliation and Quantification of Psychrophilic Sulfate-reducing Isolates in Marine Arctic Sediments

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1999 Sep 3

PMID 10473404

Citations 25

Authors

K Sahm

C Knoblauch

R Amann

Affiliations

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Abstract

Thirteen psychrophilic sulfate-reducing isolates from two permanently cold fjords of the Arctic island Spitsbergen (Hornsund and Storfjord) were phylogenetically analyzed. They all belonged to the delta subclass of Proteobacteria and were widely distributed within this group, indicating that psychrophily is a polyphyletic property. A new 16S rRNA-directed oligonucleotide probe was designed against the largest coherent cluster of these isolates. The new probe, as well as a set of available probes, was applied in rRNA slot blot hybridization to investigate the composition of the sulfate-reducing bacterial community in the sediments. rRNA related to the new cluster of incompletely oxidizing, psychrophilic isolates made up 1.4 to 20.9% of eubacterial rRNA at Storfjord and 0.6 to 3. 5% of eubacterial rRNA at Hornsund. This group was the second-most-abundant group of sulfate reducers at these sites. Denaturing gradient gel electrophoresis and hybridization analysis showed bands identical to those produced by our isolates. The data indicate that the psychrophilic isolates are quantitatively important in Svalbard sediments.

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References

Knoblauch C, Jorgensen B, Harder J . Community size and metabolic rates of psychrophilic sulfate-reducing bacteria in Arctic marine sediments. Appl Environ Microbiol. 1999; 65(9):4230-3. PMC: 99766. DOI: 10.1128/AEM.65.9.4230-4233.1999. View

Muyzer G, Teske A, Wirsen C, Jannasch H . Phylogenetic relationships of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments. Arch Microbiol. 1995; 164(3):165-72. DOI: 10.1007/BF02529967. View

Rooney-Varga J, Devereux R, Evans R, Hines M . Seasonal changes in the relative abundance of uncultivated sulfate-reducing bacteria in a salt marsh sediment and in the rhizosphere of Spartina alterniflora. Appl Environ Microbiol. 1997; 63(10):3895-901. PMC: 168699. DOI: 10.1128/aem.63.10.3895-3901.1997. View

Stahl D, Flesher B, MANSFIELD H, Montgomery L . Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Appl Environ Microbiol. 1988; 54(5):1079-84. PMC: 202606. DOI: 10.1128/aem.54.5.1079-1084.1988. View

Sahm K, MacGregor B, Jorgensen B, Stahl D . Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slot-blot hybridization in a coastal marine sediment. Environ Microbiol. 2001; 1(1):65-74. DOI: 10.1046/j.1462-2920.1999.00007.x. View

Ravenschlag K, Sahm K, Pernthaler J, Amann R . High bacterial diversity in permanently cold marine sediments. Appl Environ Microbiol. 1999; 65(9):3982-9. PMC: 99730. DOI: 10.1128/AEM.65.9.3982-3989.1999. View

Devereux , Hines , Stahl . S Cycling: Characterization of Natural Communities of Sulfate-Reducing Bacteria by 16S rRNA Sequence Comparisons. Microb Ecol. 1996; 32(3):283-92. DOI: 10.1007/BF00183063. View

Brosius J, Dull T, Sleeter D, Noller H . Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol. 1981; 148(2):107-27. DOI: 10.1016/0022-2836(81)90508-8. View

Amann R, Binder B, Olson R, Chisholm S, Devereux R, Stahl D . Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol. 1990; 56(6):1919-25. PMC: 184531. DOI: 10.1128/aem.56.6.1919-1925.1990. View

10.

Knoblauch C, Sahm K, Jorgensen B . Psychrophilic sulfate-reducing bacteria isolated from permanently cold arctic marine sediments: description of Desulfofrigus oceanense gen. nov., sp. nov., Desulfofrigus fragile sp. nov., Desulfofaba gelida gen. nov., sp. nov., Desulfotalea.... Int J Syst Bacteriol. 1999; 49 Pt 4:1631-43. DOI: 10.1099/00207713-49-4-1631. View

11.

Raskin L, Stromley J, Rittmann B, Stahl D . Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. Appl Environ Microbiol. 1994; 60(4):1232-40. PMC: 201464. DOI: 10.1128/aem.60.4.1232-1240.1994. View

12.

Friedrich M, Springer N, Ludwig W, Schink B . Phylogenetic positions of Desulfofustis glycolicus gen. nov., sp. nov., and Syntrophobotulus glycolicus gen. nov., sp. nov., two new strict anaerobes growing with glycolic acid. Int J Syst Bacteriol. 1996; 46(4):1065-9. DOI: 10.1099/00207713-46-4-1065. View

13.

Romanowski G, Lorenz M, Wackernagel W . Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils. Appl Environ Microbiol. 1993; 59(10):3438-46. PMC: 182471. DOI: 10.1128/aem.59.10.3438-3446.1993. View

14.

Lonergan D, Jenter H, Coates J, Phillips E, Schmidt T, Lovley D . Phylogenetic analysis of dissimilatory Fe(III)-reducing bacteria. J Bacteriol. 1996; 178(8):2402-8. PMC: 177952. DOI: 10.1128/jb.178.8.2402-2408.1996. View

15.

Zhou J, Bruns M, Tiedje J . DNA recovery from soils of diverse composition. Appl Environ Microbiol. 1996; 62(2):316-22. PMC: 167800. DOI: 10.1128/aem.62.2.316-322.1996. View

16.

Santegoeds , Ferdelman , Muyzer , de Beer D . Structural and functional dynamics of sulfate-reducing populations in bacterial biofilms . Appl Environ Microbiol. 1998; 64(10):3731-9. PMC: 106533. DOI: 10.1128/AEM.64.10.3731-3739.1998. View

17.

Sorensen J, Christensen D, Jorgensen B . Volatile Fatty acids and hydrogen as substrates for sulfate-reducing bacteria in anaerobic marine sediment. Appl Environ Microbiol. 1981; 42(1):5-11. PMC: 243952. DOI: 10.1128/aem.42.1.5-11.1981. View