Identification of a Universal Group B Streptococcus Vaccine by Multiple Genome Screen

Overview

Journal Science

Specialty Science

Date 2005 Jul 5

PMID 15994562

Citations 194

Authors

Domenico Maione

Immaculada Margarit

Cira D Rinaudo

Vega Masignani

Marirosa Mora

Maria Scarselli

Herve Tettelin

Cecilia Brettoni

Emilia T Iacobini

Roberto Rosini

Nunzio DAgostino

Lisa Miorin

Scilla Buccato

Massimo Mariani

Giuliano Galli

Renzo Nogarotto

Vincenzo Nardi-Dei

Vincenzo Nardi Dei

Filipo Vegni

Claire Fraser

Giuseppe Mancuso

Giuseppe Teti

Lawrence C Madoff

Lawrence C Paoletti

Rino Rappuoli

Dennis L Kasper

John L Telford

Guido Grandi

Affiliations

Soon will be listed here.

Abstract

Group B Streptococcus (GBS) is a multiserotype bacterial pathogen representing a major cause of life-threatening infections in newborns. To develop a broadly protective vaccine, we analyzed the genome sequences of eight GBS isolates and cloned and tested 312 surface proteins as vaccines. Four proteins elicited protection in mice, and their combination proved highly protective against a large panel of strains, including all circulating serotypes. Protection also correlated with antigen accessibility on the bacterial surface and with the induction of opsonophagocytic antibodies. Multigenome analysis and screening described here represent a powerful strategy for identifying potential vaccine candidates against highly variable pathogens.

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