An Improved Assay for Pyruvate Dehydrogenase in Liver and Heart

Overview

Journal Biochem J

Specialty Biochemistry

Date 1992 Jun 1

PMID 1599443

Authors

P J Randle

Affiliations

Soon will be listed here.

References

Paxton R, Sievert L . An improved assay for pyruvate dehydrogenase in liver and heart. Biochem J. 1991; 277 ( Pt 2):547-51. PMC: 1151268. DOI: 10.1042/bj2770547. View

Hutson N, Kerbey A, Randle P, Sugden P . Conversion of inactive (phosphorylated) pyruvate dehydrogenase complex into active complex by the phosphate reaction in heart mitochondria is inhibited by alloxan-diabetes or starvation in the rat. Biochem J. 1978; 173(2):669-680. PMC: 1185822. DOI: 10.1042/bj1730669. View

Sugden M, Holness M . Carbohydrate sparing and storage during the starved to fed transition. Biochem Soc Trans. 1990; 18(5):847-50. DOI: 10.1042/bst0180847. View

Beggs M, Randle P . Activity of branched-chain 2-oxo acid dehydrogenase complex in rat liver mitochondria and in rat liver. Biochem J. 1988; 256(3):929-34. PMC: 1135505. DOI: 10.1042/bj2560929. View

French T, Holness M, MacLennan P, Sugden M . Effects of nutritional status and acute variation in substrate supply on cardiac and skeletal-muscle fructose 2,6-bisphosphate concentrations. Biochem J. 1988; 250(3):773-9. PMC: 1148923. DOI: 10.1042/bj2500773. View

Holness M, Liu Y, Sugden M . Time courses of the responses of pyruvate dehydrogenase activities to short-term starvation in diaphragm and selected skeletal muscles of the rat. Biochem J. 1989; 264(3):771-6. PMC: 1133652. DOI: 10.1042/bj2640771. View

Holness M, Sugden M . Comparison of tissue pyruvate dehydrogenase activities on re-feeding rats fed ad libitum or meal-fed rats with a chow-diet meal. Biochem J. 1989; 262(1):321-5. PMC: 1133263. DOI: 10.1042/bj2620321. View

Sugden M, Holness M . Effects of re-feeding after prolonged starvation on pyruvate dehydrogenase activities in heart, diaphragm and selected skeletal muscles of the rat. Biochem J. 1989; 262(2):669-72. PMC: 1133320. DOI: 10.1042/bj2620669. View

Holness M, Sugden M . Pyruvate dehydrogenase activities during the fed-to-starved transition and on re-feeding after acute or prolonged starvation. Biochem J. 1989; 258(2):529-33. PMC: 1138393. DOI: 10.1042/bj2580529. View

10.

French T, Goode A, Holness M, MacLennan P, Sugden M . The relationship between changes in lipid fuel availability and tissue fructose 2,6-bisphosphate concentrations and pyruvate dehydrogenase complex activities in the fed state. Biochem J. 1988; 256(3):935-9. PMC: 1135506. DOI: 10.1042/bj2560935. View

11.

Patston P, Espinal J, Randle P . Effects of diet and of alloxan-diabetes on the activity of branched-chain 2-oxo acid dehydrogenase complex and of activator protein in rat tissues. Biochem J. 1984; 222(3):711-9. PMC: 1144234. DOI: 10.1042/bj2220711. View

12.

Solomon M, Cook K, Yeaman S . Effect of diet and starvation on the activity state of branched-chain 2-oxo-acid dehydrogenase complex in rat liver and heart. Biochim Biophys Acta. 1987; 931(3):335-8. DOI: 10.1016/0167-4889(87)90224-2. View

13.

French T, Goode A, Sugden M . Ischaemia and tissue pyruvate dehydrogenase activities in the rat: a comparison of the effects of cervical dislocation and pentobarbital anaesthesia. Biochem Int. 1986; 13(5):843-52. View

14.

Goodwin G, Paxton R, Gillim S, Harris R . Branched-chain 2-oxo acid dehydrogenase interferes with the measurement of the activity and activity state of hepatic pyruvate dehydrogenase. Biochem J. 1986; 236(1):111-4. PMC: 1146793. DOI: 10.1042/bj2360111. View

15.

Fatania H, Vary T, Randle P . Modulation of pyruvate dehydrogenase kinase activity in cultured hepatocytes by glucagon and n-octanoate. Biochem J. 1986; 234(1):233-6. PMC: 1146552. DOI: 10.1042/bj2340233. View

16.

Holness M, Palmer T, Sugden M . Effects of administration of tri-iodothyronine on the response of cardiac and renal pyruvate dehydrogenase complex to starvation for 48 h. Biochem J. 1985; 232(1):255-9. PMC: 1152866. DOI: 10.1042/bj2320255. View

17.

Harris R, Powell S, Paxton R, Gillim S, Nagae H . Physiological covalent regulation of rat liver branched-chain alpha-ketoacid dehydrogenase. Arch Biochem Biophys. 1985; 243(2):542-55. DOI: 10.1016/0003-9861(85)90531-4. View

18.

McCormack J, Edgell N, Denton R . Studies on the interactions of Ca2+ and pyruvate in the regulation of rat heart pyruvate dehydrogenase activity. Effects of starvation and diabetes. Biochem J. 1982; 202(2):419-27. PMC: 1158126. DOI: 10.1042/bj2020419. View

19.

Gillim S, Paxton R, Cook G, Harris R . Activity state of the branched chain alpha-ketoacid dehydrogenase complex in heart, liver, and kidney of normal, fasted, diabetic, and protein-starved rats. Biochem Biophys Res Commun. 1983; 111(1):74-81. DOI: 10.1016/s0006-291x(83)80119-3. View

20.

Caterson I, Fuller S, Randle P . Effect of the fatty acid oxidation inhibitor 2-tetradecylglycidic acid on pyruvate dehydrogenase complex activity in starved and alloxan-diabetic rats. Biochem J. 1982; 208(1):53-60. PMC: 1153928. DOI: 10.1042/bj2080053. View