Nonstimulatory Peptides Contribute to Antigen-induced CD8-T Cell Receptor Interaction at the Immunological Synapse

Overview

Journal Nat Immunol

Date 2005 Jun 28

PMID 15980863

Citations 75

Authors

Pia P Yachi

Jeanette Ampudia

Nicholas R J Gascoigne

Tomasz Zal

Affiliations

Soon will be listed here.

Abstract

It is unclear if the interaction between CD8 and the T cell receptor (TCR)-CD3 complex is constitutive or antigen induced. Here, fluorescence resonance energy transfer microscopy between fluorescent chimeras of CD3zeta and CD8beta showed that this interaction was induced by antigen recognition in the immunological synapse. Nonstimulatory endogenous or exogenous peptides presented simultaneously with antigenic peptides increased the CD8-TCR interaction. This finding indicates that the interaction between the intracellular regions of a TCR-CD3 complex recognizing its cognate peptide-major histocompatibility complex (MHC) antigen, and CD8 (plus the kinase Lck), is enhanced by a noncognate CD8-MHC interaction. Thus, the interaction of CD8 with a nonstimulatory peptide-MHC complex helps mediate T cell recognition of antigen, improving the coreceptor function of CD8.

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