Within the Cell: Analytical Techniques for Subcellular Analysis
Overview
Affiliations
This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.
A subcellular cookie cutter for spatial genomics in human tissue.
Bury A, Pyle A, Marcuccio F, Turnbull D, Vincent A, Hudson G Anal Bioanal Chem. 2022; 414(18):5483-5492.
PMID: 35233697 PMC: 9242960. DOI: 10.1007/s00216-022-03944-5.
Bioanalysis of eukaryotic organelles.
Satori C, Henderson M, Krautkramer E, Kostal V, Distefano M, Distefano M Chem Rev. 2013; 113(4):2733-811.
PMID: 23570618 PMC: 3676536. DOI: 10.1021/cr300354g.
Review on recent advances in the analysis of isolated organelles.
Satori C, Kostal V, Arriaga E Anal Chim Acta. 2012; 753:8-18.
PMID: 23107131 PMC: 3484375. DOI: 10.1016/j.aca.2012.09.041.
Sampling techniques for single-cell electrophoresis.
Cecala C, Sweedler J Analyst. 2012; 137(13):2922-9.
PMID: 22288071 PMC: 3558037. DOI: 10.1039/c2an16211c.
The subcellular distribution of small molecules: from pharmacokinetics to synthetic biology.
Zheng N, Tsai H, Zhang X, Rosania G Mol Pharm. 2011; 8(5):1619-28.
PMID: 21805990 PMC: 3185113. DOI: 10.1021/mp200092v.