Genomic Analysis of Drosophila Chromosome Underreplication Reveals a Link Between Replication Control and Transcriptional Territories

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2005 Jun 2

PMID 15928082

Citations 49

Authors

Stepan N Belyakin

George K Christophides

Artyom A Alekseyenko

Evgenia V Kriventseva

Elena S Belyaeva

Roman A Nanayev

Igor V Makunin

Fotis C Kafatos

Igor F Zhimulev

Affiliations

Soon will be listed here.

Abstract

In Drosophila polytene chromosomes, most late-replicating regions remain underreplicated. A loss-of-function mutant of the suppressor of underreplication [Su(UR)] gene suppresses underreplication (UR), whereas extra copies of this gene enhance the level and number of regions showing UR. By combining DNA microarray analysis with manipulation of the number of Su(UR) gene copies, we achieved genomic-scale molecular identification of 1,036 genes that are arranged in clusters located in 52 UR chromosomal regions. These regions overlap extensively (96%) but are not completely identical with late-replicating regions of mitotically dividing Kc cells in culture. Reanalysis of published gene expression profiles revealed that genomic regions defined by replication properties include clusters of coordinately expressed genes. Genomic regions that are UR in polytene chromosomes and late replicated in Kc cell chromosomes show a particularly common association with transcriptional territories that are expressed in testis/males but not ovary/females or embryos. An attractive hypothesis for future testing is that factors involved in replication control, such as SU(UR), may interact physically with those involved in epigenetic silencing of transcription territories.

Citing Articles

The Organization of Pericentromeric Heterochromatin in Polytene Chromosome 3 of the Line with the Mutations Suppressing Underreplication.

Zykova T, Maltseva M, Goncharov F, Boldyreva L, Pokholkova G, Kolesnikova T Cells. 2021; 10(11).

PMID: 34831030 PMC: 8616060. DOI: 10.3390/cells10112809.

Effects of Mutations in the Gene on the Replication and Underreplication of Pericentromeric Heterochromatin in Salivary Gland Polytene Chromosomes.

Kolesnikova T, Kolodyazhnaya A, Pokholkova G, Schubert V, Dovgan V, Romanenko S Cells. 2020; 9(6).

PMID: 32575592 PMC: 7349278. DOI: 10.3390/cells9061501.

Faint gray bands in Drosophila melanogaster polytene chromosomes are formed by coding sequences of housekeeping genes.

Demakova O, Demakov S, Boldyreva L, Zykova T, Levitsky V, Semeshin V Chromosoma. 2019; 129(1):25-44.

PMID: 31820086 DOI: 10.1007/s00412-019-00728-2.

Gene dunce Localization in the Polytene Chromosome of Drosophila melanogaster Long Span Batch of Adjacent Chromosomal Structures.

Khoroshko V, Pokholkova G, Zykova T, Osadchiy I, Zhimulev I Dokl Biochem Biophys. 2019; 484(1):55-58.

PMID: 31012014 DOI: 10.1134/S1607672919010137.

Polytene Chromosomes - A Portrait of Functional Organization of the Genome.

Zykova T, Levitsky V, Belyaeva E, Zhimulev I Curr Genomics. 2018; 19(3):179-191.

PMID: 29606905 PMC: 5850506. DOI: 10.2174/1389202918666171016123830.

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