Stable T Cell-dendritic Cell Interactions Precede the Development of Both Tolerance and Immunity in Vivo

Overview

Journal Nat Immunol

Date 2005 Jun 1

PMID 15924144

Citations 139

Authors

Guy Shakhar

Randall L Lindquist

Dimitris Skokos

Diana Dudziak

Julie H Huang

Michel C Nussenzweig

Michael L Dustin

Affiliations

Soon will be listed here.

Abstract

The maturation status of dendritic cells (DCs) determines whether they prime or tolerize T cells. We targeted ovalbumin peptide exclusively to DCs in situ using an antibody to DEC-205 and studied the interaction of DCs with naive CD4(+) T cells in tolerizing or priming conditions. We used two-photon microscopy to simultaneously track antigen-specific OT-II T cells, nonspecific T cells and DCs in lymph nodes of living mice. In both tolerance and immunity, OT-II cells arrested on DCs near high endothelial venules beginning shortly after extravasation and regained their baseline speed by 18 h. Thus, early antigen-dependent T cell arrest on DCs is a shared feature of tolerance and priming associated with activation and proliferation.

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