Growth of LNCaP Cells in Monoculture and Coculture with Osteoblasts and Response to TNOX Inhibitors

An in vitro coculture model of prostate cancer cells (LNCaP) with human osteoblasts (hFOB) was utilized to define the efficacy of the tNOX inhibitors EGCg, capsaicin, Capsibiol-T and phenoxodiol against bone metastasis of prostate cancer alone and in combination with Taxol and cisplatin. In general, the LNCaP cells were more resistant to treatment with EGCg, capsaicin, phenoxodiol and Taxol when grown in coculture than when grown in monoculture. Only with Capsibiol-T (50 microM) was growth of LNCaP cells in coculture inhibited comparable with monoculture. Pretreatment with Capsibiol-T followed by the treatment with Taxol had an additive effect on reduction of viability of LNCaP cells in monoculture. In contrast, an antagonistic effect of cisplatin was observed following capsaicin pretreatment.