Bacteriophage Mu Targets the Trinucleotide Sequence CGG

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2005 May 4

PMID 15866949

Citations 12

Authors

Dipankar Manna

Shuang Deng

Adam M Breier

N Patrick Higgins

Affiliations

Soon will be listed here.

Abstract

Target specificity for bacteriophage Mu was studied using a new phage derivative that enabled cloning of Mu-host junctions from phage DNA. Insertions distributed throughout the chromosome showed no orientation bias with respect to transcription or replication polarity. Genes with a high frequency of the triplet CGG were preferred targets.

Citing Articles

Pseudotyping Bacteriophage P2 Tail Fibers to Extend the Host Range for Biomedical Applications.

Cunliffe T, Parker A, Jaramillo A ACS Synth Biol. 2022; 11(10):3207-3215.

PMID: 36084285 PMC: 9594776. DOI: 10.1021/acssynbio.1c00629.

A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction.

Ho T, Shao A, Lu Z, Savilahti H, Menolascina F, Wang L Nat Commun. 2021; 12(1):2200.

PMID: 33850130 PMC: 8044194. DOI: 10.1038/s41467-021-22404-9.

Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of .

Walker D, Harshey R Mob DNA. 2020; 11:26.

PMID: 32670425 PMC: 7350765. DOI: 10.1186/s13100-020-00217-9.

Targeted insertional mutagenesis libraries for deep domain insertion profiling.

Coyote-Maestas W, Nedrud D, Okorafor S, He Y, Schmidt D Nucleic Acids Res. 2019; 48(2):e11.

PMID: 31745561 PMC: 6954442. DOI: 10.1093/nar/gkz1110.

Diversity of transducer-like proteins (Tlps) in Campylobacter.

Clark C, Berry C, Demczuk W PLoS One. 2019; 14(3):e0214228.

PMID: 30908544 PMC: 6433261. DOI: 10.1371/journal.pone.0214228.

References

Yu D, Ellis H, Lee E, Jenkins N, Copeland N, Court D . An efficient recombination system for chromosome engineering in Escherichia coli. Proc Natl Acad Sci U S A. 2000; 97(11):5978-83. PMC: 18544. DOI: 10.1073/pnas.100127597. View

Jones J, Welty D, Nakai H . Versatile action of Escherichia coli ClpXP as protease or molecular chaperone for bacteriophage Mu transposition. J Biol Chem. 1998; 273(1):459-65. DOI: 10.1074/jbc.273.1.459. View

Peters J, Craig N . Tn7 transposes proximal to DNA double-strand breaks and into regions where chromosomal DNA replication terminates. Mol Cell. 2000; 6(3):573-82. DOI: 10.1016/s1097-2765(00)00056-3. View

Peters J, Craig N . Tn7 recognizes transposition target structures associated with DNA replication using the DNA-binding protein TnsE. Genes Dev. 2001; 15(6):737-47. PMC: 312648. DOI: 10.1101/gad.870201. View

Manna D, Wang X, Higgins N . Mu and IS1 transpositions exhibit strong orientation bias at the Escherichia coli bgl locus. J Bacteriol. 2001; 183(11):3328-35. PMC: 99630. DOI: 10.1128/JB.183.11.3328-3335.2001. View

Greene E, Mizuuchi K . Direct observation of single MuB polymers: evidence for a DNA-dependent conformational change for generating an active target complex. Mol Cell. 2002; 9(5):1079-89. DOI: 10.1016/s1097-2765(02)00514-2. View

Paulsen I, Banerjei L, Myers G, Nelson K, Seshadri R, Read T . Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 2003; 299(5615):2071-4. DOI: 10.1126/science.1080613. View

Canchaya C, Fournous G, Brussow H . The impact of prophages on bacterial chromosomes. Mol Microbiol. 2004; 53(1):9-18. DOI: 10.1111/j.1365-2958.2004.04113.x. View

Manna D, Breier A, Higgins N . Microarray analysis of transposition targets in Escherichia coli: the impact of transcription. Proc Natl Acad Sci U S A. 2004; 101(26):9780-5. PMC: 470751. DOI: 10.1073/pnas.0400745101. View

10.

Pato M . Replication of Mu prophages lacking the central strong gyrase site. Res Microbiol. 2004; 155(7):553-8. DOI: 10.1016/j.resmic.2004.03.006. View

11.

Hattman S . Specificity of the bacteriophage Mu mom+ -controlled DNA modification. J Virol. 1980; 34(1):277-9. PMC: 288695. DOI: 10.1128/JVI.34.1.277-279.1980. View

12.

Emr S, Silhavy T . Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage lambda receptor. J Mol Biol. 1980; 141(1):63-90. DOI: 10.1016/s0022-2836(80)80029-5. View

13.

Mizuuchi K . Transpositional recombination: mechanistic insights from studies of mu and other elements. Annu Rev Biochem. 1992; 61:1011-51. DOI: 10.1146/annurev.bi.61.070192.005051. View

14.

Baker T, Mizuuchi K . DNA-promoted assembly of the active tetramer of the Mu transposase. Genes Dev. 1992; 6(11):2221-32. DOI: 10.1101/gad.6.11.2221. View

15.

Kruklitis R, Nakai H . Participation of the bacteriophage Mu A protein and host factors in the initiation of Mu DNA synthesis in vitro. J Biol Chem. 1994; 269(23):16469-77. View

16.

Wang X, Higgins N . 'Muprints' of the lac operon demonstrate physiological control over the randomness of in vivo transposition. Mol Microbiol. 1994; 12(4):665-77. DOI: 10.1111/j.1365-2958.1994.tb01054.x. View

17.

Mizuuchi M, Mizuuchi K . Target site selection in transposition of phage Mu. Cold Spring Harb Symp Quant Biol. 1993; 58:515-23. DOI: 10.1101/sqb.1993.058.01.058. View

18.

Chaconas G, Lavoie B, Watson M . DNA transposition: jumping gene machine, some assembly required. Curr Biol. 1996; 6(7):817-20. DOI: 10.1016/s0960-9822(02)00603-6. View

19.

Blattner F, Plunkett 3rd G, Bloch C, Perna N, Burland V, Riley M . The complete genome sequence of Escherichia coli K-12. Science. 1997; 277(5331):1453-62. DOI: 10.1126/science.277.5331.1453. View

20.

Datsenko K, Wanner B . One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000; 97(12):6640-5. PMC: 18686. DOI: 10.1073/pnas.120163297. View