Target Accessibility Dictates the Potency of Human RISC

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2005 Apr 27

PMID 15852021

Citations 61

Authors

Kirk M Brown

Chia-Ying Chu

Tariq M Rana

Affiliations

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Abstract

In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC(*)) catalysis. Kinetic studies revealed that siRNA-programmed RISC(*) cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC(*) catalysis.

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