Efficient Formation of Bipolar Microtubule Bundles Requires Microtubule-bound Gamma-tubulin Complexes

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2005 Apr 20

PMID 15837798

Citations 65

Authors

Marcel E Janson

Thanuja Gangi Setty

Anne Paoletti

P T Tran

Affiliations

Soon will be listed here.

Abstract

The mechanism for forming linear microtubule (MT) arrays in cells such as neurons, polarized epithelial cells, and myotubes is not well understood. A simpler bipolar linear array is the fission yeast interphase MT bundle, which in its basic form contains two MTs that are bundled at their minus ends. Here, we characterize mto2p as a novel fission yeast protein required for MT nucleation from noncentrosomal gamma-tubulin complexes (gamma-TuCs). In interphase mto2Delta cells, MT nucleation was strongly inhibited, and MT bundling occurred infrequently and only when two MTs met by chance in the cytoplasm. In wild-type 2, we observed MT nucleation from gamma-TuCs bound along the length of existing MTs. We propose a model on how these nucleation events can more efficiently drive the formation of bipolar MT bundles in interphase. Key to the model is our observation of selective antiparallel binding of MTs, which can both explain the generation and spatial separation of multiple bipolar bundles.

Citing Articles

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