Proteomic Identification of Ku70/Ku80 Autoantigen Recognized by Monoclonal Antibody Against Hepatocellular Carcinoma

Overview

Journal Proteomics

Date 2005 Apr 16

PMID 15832367

Citations 6

Authors

John M Luk

Yvonne C F Su

Stanley C T Lam

Carol K Lee

Mei Yu Hu

Qing Yu He

George K Lau

Fred W S Wong

Sheung-Tat Fan

Affiliations

Soon will be listed here.

Abstract

A murine monoclonal antibody (mAb), CLD3 (IgG(1),kappa), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix-assisted laser desorption/ionization-time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.

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