Mapping Sites of Protein Phosphorylation by Mass Spectrometry Utilizing a Chemical-enzymatic Approach: Characterization of Products from Alpha-S1 Casein Phosphopeptides

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2005 Apr 13

PMID 15822919

Citations 1

Authors

Daniel J McCormick

Michael W Holmes

David C Muddiman

Benjamin J Madden

Affiliations

Soon will be listed here.

Abstract

A novel chemical-enzymatic approach was developed to facilitate identification of phosphorylation sites in isolated phosphoproteins. ESI-TOF mass spectrometry was used to characterize products from the chemical-enzymatic cleavage of specific phosphorylation sites in bovine alpha-S1 casein and synthetic phosphopeptides containing substitutions at a single phosphorylation site. Further refinements to this approach for identification of protein phosphorylation sites and its utility for the quantification of phosphopeptides by isotope-dilution mass spectrometry are presented.

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PMID: 21110323 PMC: 3021470. DOI: 10.1002/pmic.201000080.

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