Gene Expression in Porphyromonas Gingivalis After Contact with Human Epithelial Cells

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2005 Mar 24

PMID 15784578

Citations 34

Authors

Yumiko Hosogi

Margaret J Duncan

Affiliations

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Abstract

Porphyromonas gingivalis, a gram-negative oral anaerobe, is strongly associated with adult periodontitis. The adherence of the organism to host epithelium signals changes in both cell types as bacteria initiate infection and colonization and epithelial cells rally their defenses. We hypothesized that the expression of a defined set of P. gingivalis genes would be consistently up-regulated during infection of HEp-2 human epithelial cells. P. gingivalis genome microarrays were used to compare the gene expression profiles of bacteria that adhered to HEp-2 cells and bacteria that were incubated alone. Genes whose expression was temporally up-regulated included those involved in the oxidative stress response and those encoding heat shock proteins that are essential to maintaining cell viability under adverse conditions. The results suggest that contact with epithelial cells induces in P. gingivalis stress-responsive pathways that promote the survival of the bacterium.

Citing Articles

The Oxidative Stress-Induced Hypothetical Protein PG_0686 in Porphyromonas gingivalis W83 Is a Novel Diguanylate Cyclase.

Ximinies A, Dou Y, Mishra A, Zhang K, Deivanayagam C, Wang C Microbiol Spectr. 2023; :e0441122.

PMID: 36719196 PMC: 10101095. DOI: 10.1128/spectrum.04411-22.

Secretome of Paenibacillus sp. S-12 provides an insight about its survival and possible pathogenicity.

Kumari K, Sharma P, Ma Y, Singh R Folia Microbiol (Praha). 2023; 68(4):559-569.

PMID: 36642775 DOI: 10.1007/s12223-023-01032-4.

Contribution of -Omics Technologies in the Study of during Periodontitis Pathogenesis: A Minireview.

Nunez-Belmar J, Morales-Olavarria M, Vicencio E, Vernal R, Cardenas J, Cortez C Int J Mol Sci. 2023; 24(1).

PMID: 36614064 PMC: 9820714. DOI: 10.3390/ijms24010620.

Secretome analysis of an environmental isolate Enterobacter sp. S-33 identifies proteins related to pathogenicity.

Kumari K, Sharma P, Aggarwal Y, Singh R Arch Microbiol. 2022; 204(11):662.

PMID: 36198868 DOI: 10.1007/s00203-022-03277-y.

Gene expression of Porphyromonas gingivalis ATCC 33277 when growing in an in vitro multispecies biofilm.

Romero-Lastra P, Sanchez M, Llama-Palacios A, Figuero E, Herrera D, Sanz M PLoS One. 2019; 14(8):e0221234.

PMID: 31437202 PMC: 6706054. DOI: 10.1371/journal.pone.0221234.

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