Can Changes in Hydrophobicity Increase the Bioavailability of Alpha-tocopherol?

Overview

Journal Eur J Nutr

Specialty Nutritional Sciences

Date 2005 Mar 15

PMID 15765200

Citations 3

Authors

Evelyn I Back

Claudia Frindt

Erika Ocenaskova

Donatus Nohr

Martin Stern

Hans K Biesalski

Affiliations

Soon will be listed here.

Abstract

Background: Bioavailability of fat-soluble vitamins from conventional oral supplements is insufficient in some conditions in which fat digestion and absorption are chronically impaired (e. g. cystic fibrosis).

Aim Of The Study: We used a water-soluble form of fatsoluble vitamin E (AQUANOVA solubilisate) to create a nutritional supplement (NS) in the form of vitaminized gummi bears (with micellised water-soluble alpha-tocopheryl acetate (100 IU) and 400 mg crystalline vitamin C). We assessed the bioavailability of the NS in comparison to conventional preparations.

Methods: The trial consisted of three study days (d0: NS sucked; d10: NS swallowed; d20: reference products swallowed). A total of 14 subjects (6 male/8 female), aged 25.3 (22.7-35.3) years, BMI 24.3 (19.0-31.7) kg/m(2) participated in the study. They had blood samples drawn after fasting for >or=12 hours and then 1, 5, 15, 30, 60, 120, 180, 240, 300 and 320 minutes after ingesting the vitamins. HPLC and a colorimetric method were used to determine vitamin E and vitamin C, respectively. Areas under the curve (AUC(0-320min)) and maximum increases in plasma concentrations (Delta concentration) were calculated to assess bioavailability.

Results: The AUCs(0-320min) of alpha-tocopherol from d0 were significantly larger (p = 0.016) when compared to d20. Moreover, the maximum increase in alpha-tocopherol plasma concentrations was significantly higher for d0 (p = 0.023) and d10 (p = 0.002) when compared to d20.

Conclusions: Short-term bioavailability of AQUANOVA micellised fat-soluble vitamin E from our NS was significantly higher than from regular supplements. The NS will now be tested for its clinical efficacy in a randomized double-blind controlled intervention trial with CF patients.

Citing Articles

Application of Nanomicelles in Enhancing Bioavailability and Biological Efficacy of Bioactive Nutrients.

Li L, Zeng Y, Chen M, Liu G Polymers (Basel). 2022; 14(16).

PMID: 36015535 PMC: 9415603. DOI: 10.3390/polym14163278.

Oral Supplementation with a Special Additive of Retinyl Palmitate and Alpha Tocopherol Reduces Growth Retardation in Young Pancreatic Duct Ligated Pigs Used as a Model for Children Suffering from Exocrine Pancreatic Insufficiency.

Mosseler A, Schmicke M, Holtershinken M, Beyerbach M, Kamphues J Int J Mol Sci. 2016; 17(10).

PMID: 27690005 PMC: 5085675. DOI: 10.3390/ijms17101642.

Altered bacterial metabolism, not coenzyme Q content, is responsible for the lifespan extension in Caenorhabditis elegans fed an Escherichia coli diet lacking coenzyme Q.

Saiki R, Lunceford A, Bixler T, Dang P, Lee W, Furukawa S Aging Cell. 2008; 7(3):291-304.

PMID: 18267002 PMC: 3104051. DOI: 10.1111/j.1474-9726.2008.00378.x.

References

Sokol R, Heubi J, Iannaccone S, McClung H, Accurso F, Hammond K . Vitamin E deficiency neuropathy in children with fat malabsorption. Studies in cystic fibrosis and chronic cholestasis. Ann N Y Acad Sci. 1989; 570:156-69. DOI: 10.1111/j.1749-6632.1989.tb14916.x. View

Gronowitz E, Andersson H, Strandvik B . Water-miscible tocopherol is not superior to fat-soluble preparation for vitamin E absorption in cystic fibrosis. Acta Paediatr. 2001; 90(10):1112-5. DOI: 10.1080/080352501317061486. View

Sokol R, Heubi J, McClung H, Lilly J, Silverman A . Treatment of vitamin E deficiency during chronic childhood cholestasis with oral d-alpha-tocopheryl polyethylene glycol-1000 succinate. Gastroenterology. 1987; 93(5):975-85. DOI: 10.1016/0016-5085(87)90559-2. View

Traber M, KAYDEN H, Green J, Green M . Absorption of water-miscible forms of vitamin E in a patient with cholestasis and in thoracic duct-cannulated rats. Am J Clin Nutr. 1986; 44(6):914-23. DOI: 10.1093/ajcn/44.6.914. View

HARRIES J, Muller D . Absorption of different doses of fat soluble and water miscible preparations of vitamin E in children with cystic fibrosis. Arch Dis Child. 1971; 46(247):341-4. PMC: 1647675. DOI: 10.1136/adc.46.247.341. View

Lancellotti L, DOrazio C, Mastella G, Mazzi G, Lippi U . Deficiency of vitamins E and A in cystic fibrosis is independent of pancreatic function and current enzyme and vitamin supplementation. Eur J Pediatr. 1996; 155(4):281-5. DOI: 10.1007/BF02002713. View

Traber M, Schiano T, Steephen A, KAYDEN H, Shike M . Efficacy of water-soluble vitamin E in the treatment of vitamin E malabsorption in short-bowel syndrome. Am J Clin Nutr. 1994; 59(6):1270-4. DOI: 10.1093/ajcn/59.6.1270. View

Ihara H, Matsumoto N, Shino Y, Aoki Y, Hashizume N, Nanba S . An automated assay for measuring serum ascorbic acid with use of 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy, free radical and o-phenylenediamine. Clin Chim Acta. 2000; 301(1-2):193-204. DOI: 10.1016/s0009-8981(00)00356-9. View

Bateman N, Uccellini D . Effect of formulation on the bioavailability of retinol, D-alpha-tocopherol and riboflavine. J Pharm Pharmacol. 1984; 36(7):461-4. DOI: 10.1111/j.2042-7158.1984.tb04426.x. View

10.

Bateman N, Uccellini D . Kinetics of D-alpha-tocopherol in a water soluble base in man. J Pharm Pharmacol. 1985; 37(10):728-9. DOI: 10.1111/j.2042-7158.1985.tb04952.x. View

11.

Ihara H, Shino Y, Aoki Y, Hashizume N, Minegishi N . A simple and rapid method for the routine assay of total ascorbic acid in serum and plasma using ascorbate oxidase and o-phenylenediamine. J Nutr Sci Vitaminol (Tokyo). 2001; 46(6):321-4. DOI: 10.3177/jnsv.46.321. View

12.

Back E, Frindt C, Nohr D, Frank J, Ziebach R, Stern M . Antioxidant deficiency in cystic fibrosis: when is the right time to take action?. Am J Clin Nutr. 2004; 80(2):374-84. DOI: 10.1093/ajcn/80.2.374. View

13.

Traber M, Thellman C, Rindler M, KAYDEN H . Uptake of intact TPGS (d-alpha-tocopheryl polyethylene glycol 1000 succinate) a water-miscible form of vitamin E by human cells in vitro. Am J Clin Nutr. 1988; 48(3):605-11. DOI: 10.1093/ajcn/48.3.605. View

14.

Winklhofer-Roob B, vant Hof M, SHMERLING D . Long-term oral vitamin E supplementation in cystic fibrosis patients: RRR-alpha-tocopherol compared with all-rac-alpha-tocopheryl acetate preparations. Am J Clin Nutr. 1996; 63(5):722-8. DOI: 10.1093/ajcn/63.5.722. View