Induction of Cancer-specific Cytotoxicity Towards Human Prostate and Skin Cells Using Quercetin and Ultrasound

Overview

Journal Br J Cancer

Specialty Oncology

Date 2005 Feb 3

PMID 15685239

Citations 14

Authors

S Paliwal

J Sundaram

S Mitragotri

Affiliations

Soon will be listed here.

Abstract

Bioflavonoids, such as quercetin, have recently emerged as a new class of chemotherapeutic drugs for the treatment of various cancer types, but are marred by their low potency and poor selectivity. We report that a short application of low-frequency ultrasound selectively sensitises prostate and skin cancer cells against quercetin. Pretreatment of cells with ultrasound (20 kHz, 2 W cm(-2), 60 s) selectively induced cytotoxicity in skin and prostate cancer cells, while having minimal effect on corresponding normal cell lines. About 90% of the viable skin cancer cell population was lost within 48 h after ultrasound-quercetin (50 microM) treatment. Ultrasound reduced the LC50 of quercetin for skin cancer cells by almost 80-fold, while showing no effect on LC50 for nonmalignant skin cells.

Citing Articles

A recent update on the connection between dietary phytochemicals and skin cancer: emerging understanding of the molecular mechanism.

Singh H, Mishra A, Mohanto S, Kumar A, Mishra A, Amin R Ann Med Surg (Lond). 2024; 86(10):5877-5913.

PMID: 39359831 PMC: 11444613. DOI: 10.1097/MS9.0000000000002392.

Effects of Quercetin and Curcumin Combination on Antibacterial, Antioxidant, In Vitro Wound Healing and Migration of Human Dermal Fibroblast Cells.

Chittasupho C, Manthaisong A, Okonogi S, Tadtong S, Samee W Int J Mol Sci. 2022; 23(1).

PMID: 35008566 PMC: 8745450. DOI: 10.3390/ijms23010142.

Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.

Stewart M, Langer R, Jensen K Chem Rev. 2018; 118(16):7409-7531.

PMID: 30052023 PMC: 6763210. DOI: 10.1021/acs.chemrev.7b00678.

Imaging techniques: new avenues in cancer gene and cell therapy.

Saadatpour Z, Rezaei A, Ebrahimnejad H, Baghaei B, Bjorklund G, Chartrand M Cancer Gene Ther. 2016; 24(1):1-5.

PMID: 27834357 DOI: 10.1038/cgt.2016.61.

Chemopreventive Agents and Inhibitors of Cancer Hallmarks: May Citrus Offer New Perspectives?.

Cirmi S, Ferlazzo N, Lombardo G, Maugeri A, Calapai G, Gangemi S Nutrients. 2016; 8(11.

PMID: 27827912 PMC: 5133085. DOI: 10.3390/nu8110698.

References

Carter P . Improving the efficacy of antibody-based cancer therapies. Nat Rev Cancer. 2002; 1(2):118-29. DOI: 10.1038/35101072. View

BURDON R . Thermotolerance and the heat shock proteins. Symp Soc Exp Biol. 1987; 41:269-83. View

Salucci M, Stivala L, Maiani G, Bugianesi R, Vannini V . Flavonoids uptake and their effect on cell cycle of human colon adenocarcinoma cells (Caco2). Br J Cancer. 2002; 86(10):1645-51. PMC: 2746583. DOI: 10.1038/sj.bjc.6600295. View

Kiang J, Tsokos G . Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. Pharmacol Ther. 1998; 80(2):183-201. DOI: 10.1016/s0163-7258(98)00028-x. View

Choi J, Kim J, Lee J, Kang C, Kwon H, Yoo Y . Induction of cell cycle arrest and apoptosis in human breast cancer cells by quercetin. Int J Oncol. 2001; 19(4):837-44. DOI: 10.3892/ijo.19.4.837. View

Nakanoma T, Ueno M, Iida M, Hirata R, Deguchi N . Effects of quercetin on the heat-induced cytotoxicity of prostate cancer cells. Int J Urol. 2002; 8(11):623-30. DOI: 10.1046/j.1442-2042.2001.00389.x. View

Lee S, Kuan C, Yang C, Yang S . Bioflavonoids commonly and potently induce tyrosine dephosphorylation/inactivation of oncogenic proline-directed protein kinase FA in human prostate carcinoma cells. Anticancer Res. 1998; 18(2A):1117-21. View

Kobayashi T, Nakata T, Kuzumaki T . Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Lett. 2002; 176(1):17-23. DOI: 10.1016/s0304-3835(01)00738-8. View

Gan Y, Au J, Lu J, Wientjes M . Antiproliferative and cytotoxic effects of geldanamycin, cytochalasin E, suramin and thiacetazone in human prostate xenograft tumor histocultures. Pharm Res. 1998; 15(11):1760-6. DOI: 10.1023/a:1011921031564. View

10.

Jaattela M . Escaping cell death: survival proteins in cancer. Exp Cell Res. 1999; 248(1):30-43. DOI: 10.1006/excr.1999.4455. View

11.

Lynch T, Bell D, Sordella R, Gurubhagavatula S, Okimoto R, Brannigan B . Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. N Engl J Med. 2004; 350(21):2129-39. DOI: 10.1056/NEJMoa040938. View

12.

Hosokawa N, HIRAYOSHI K, Nakai A, Hosokawa Y, Marui N, Yoshida M . Flavonoids inhibit the expression of heat shock proteins. Cell Struct Funct. 1990; 15(6):393-401. DOI: 10.1247/csf.15.393. View

13.

Guillemard V, Saragovi H . Novel approaches for targeted cancer therapy. Curr Cancer Drug Targets. 2004; 4(4):313-26. DOI: 10.2174/1568009043332989. View

14.

Elia G, Santoro M . Regulation of heat shock protein synthesis by quercetin in human erythroleukaemia cells. Biochem J. 1994; 300 ( Pt 1):201-9. PMC: 1138143. DOI: 10.1042/bj3000201. View

15.

Chan M, Fong D, Soprano K, Holmes W, Heverling H . Inhibition of growth and sensitization to cisplatin-mediated killing of ovarian cancer cells by polyphenolic chemopreventive agents. J Cell Physiol. 2002; 194(1):63-70. DOI: 10.1002/jcp.10186. View

16.

Selkirk J, He C, Patterson R, Merrick B . Tumor suppressor p53 gene forms multiple isoforms: evidence for single locus origin and cytoplasmic complex formation with heat shock proteins. Electrophoresis. 1996; 17(11):1764-71. DOI: 10.1002/elps.1150171114. View

17.

Brown J, Khodr H, Hider R, Rice-Evans C . Structural dependence of flavonoid interactions with Cu2+ ions: implications for their antioxidant properties. Biochem J. 1998; 330 ( Pt 3):1173-8. PMC: 1219258. DOI: 10.1042/bj3301173. View

18.

Mendelsohn J, Baselga J . The EGF receptor family as targets for cancer therapy. Oncogene. 2001; 19(56):6550-65. DOI: 10.1038/sj.onc.1204082. View

19.

Li D, Li Calzi S, Sanchez E . Inhibition of heat shock factor activity prevents heat shock potentiation of glucocorticoid receptor-mediated gene expression. Cell Stress Chaperones. 1999; 4(4):223-34. PMC: 312937. DOI: 10.1379/1466-1268(1999)004<0223:iohsfa>2.3.co;2. View

20.

Jolly C, Morimoto R . Role of the heat shock response and molecular chaperones in oncogenesis and cell death. J Natl Cancer Inst. 2000; 92(19):1564-72. DOI: 10.1093/jnci/92.19.1564. View