PR-Set7-dependent Methylation of Histone H4 Lys 20 Functions in Repression of Gene Expression and is Essential for Mitosis

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2005 Feb 1

PMID 15681608

Citations 101

Authors

Dmitry Karachentsev

Kavitha Sarma

Danny Reinberg

Ruth Steward

Affiliations

Soon will be listed here.

Abstract

The histone methyl transferase PR-Set7 mediates histone H4 Lys 20 methylation, a mark of constitutive and facultative heterochromatin. We isolated a null mutation in Drosophila PR-Set7 that suppresses position effect variegation, indicating that PR-Set7 indeed functions in silencing general gene expression. In PR-Set7 larval leg and eye discs, the number of cells is lower than normal, and the DNA content in these cells is significantly increased. These data show that PR-Set7-dependent methylation is essential for the process of mitosis. The methylation mark is highly stable and is maintained even in the absence of PR-Set7 protein.

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