Existence and Significance of Hepatitis B Virus DNA in Kidneys of IgA Nephropathy

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2005 Jan 19

PMID 15655828

Citations 11

Authors

Nian-Song Wang

Zhao-Long Wu

Yue-E Zhang

Lu-Tan Liao

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the existence and significance of hepatitis B virus (HBV) DNA in the pathogenesis of IgA nephropathy (IgAN).

Methods: Fifty cases of IgAN with HBV antigenaemia and/or hepatitis B virus antigens (HBAg, or HBsAg, HBcAg) detected by immunohistochemistry in renal tissues were enrolled in our study. The distribution and localization of HBV DNA were observed using in situ hybridization. Southern blot analysis was performed to reveal the state of renal HBV DNA.

Results: Among the 50 patients with IgAN, HBs antigenemia was detected in 17 patients (34%). HBAg in renal tissues was detected in 48 patients (96%), the positive rate of HBAg, HBsAg, and HBcAg was 82% (41/50), 58% (29/50), and 42% (21/50) in glomeruli, respectively; and was 94% (47/50), 56% (28/50) and 78% (39/50) in tubular epithelia, respectively. Positive HBV DNA was detected in 72% (36/50) and 82% (41/50) cases in tubular epithelia and glomeruli respectively by in situ hybridization, and the positive signals were localized in the nuclei of tubular epithelial cells and glomerular mesangial cells as well as infiltrated interstitial lymphocytes. Moreover, 68% (34/50) cases were proved to be HBV DNA positive by Southern blot analysis, and all were the integrated form.

Conclusion: HBV infection might play an important role in occurrence and progress of IgAN. In addition to humoral immune damages mediated by HBAg-HBAb immune complex, renal tissues of some IgAN are directly infected with HBV and express HBAg in situ, and the cellular mechanism mediated by HBV originating from renal cells in situ may also be involved in the pathogenesis of IgAN.

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References

Zhang Y, Guo M, Ying Y . [Further study on the immunopathology of hepatitis B virus associated glomerulonephritis]. Zhonghua Nei Ke Za Zhi. 1990; 29(9):526-9, 574. View

Endo Y, Kanbayashi H . Etiology of IgA nephropathy syndrome. Pathol Int. 1994; 44(1):1-13. DOI: 10.1111/j.1440-1827.1994.tb02579.x. View

Thyagarajan S, Thirunalasundari T, Subramanian S, Panchanadam M, Nammalwar B, Prabha V . Serum and tissue positivity for hepatitis B virus markers in histopathologically proven glomerulonephropathies. J Med Microbiol. 1989; 29(4):243-9. DOI: 10.1099/00222615-29-4-243. View

Wang N, Wu Z, Zhang Y, Guo M, Liao L . Role of hepatitis B virus infection in pathogenesis of IgA nephropathy. World J Gastroenterol. 2003; 9(9):2004-8. PMC: 4656662. DOI: 10.3748/wjg.v9.i9.2004. View

Zhang Y, Fang L, Ma X . [Hepatitis B virus infection and pathogenesis of glomerulonephritis]. Zhonghua Bing Li Xue Za Zhi. 1995; 24(6):341-4. View

Lin C . Hepatitis B virus deoxyribonucleic acid in kidney cells probably leading to viral pathogenesis among hepatitis B virus associated membranous nephropathy patients. Nephron. 1993; 63(1):58-64. DOI: 10.1159/000187144. View

Hirose H, Udo K, Kojima M, Takahashi Y, Miyakawa Y, Miyamoto K . Deposition of hepatitis B e antigen in membranous glomerulonephritis: identification by F(ab')2 fragments of monoclonal antibody. Kidney Int. 1984; 26(3):338-41. DOI: 10.1038/ki.1984.178. View

Araki K, Miyazaki J, Hino O, Tomita N, Chisaka O, Matsubara K . Expression and replication of hepatitis B virus genome in transgenic mice. Proc Natl Acad Sci U S A. 1989; 86(1):207-11. PMC: 286433. DOI: 10.1073/pnas.86.1.207. View

Yu Y, Wang H, Chen M . [An in situ hybridization and immunofluorescent double staining study on the pathogenesis of HBV in kidney disease]. Zhonghua Nei Ke Za Zhi. 1990; 29(9):538-40, 575. View

10.

Furuse A, Hattori S, Terashima T, Karashima S, Matsuda I . Circulating immune complex in glomerulonephropathy associated with hepatitis B virus infection. Nephron. 1982; 31(3):212-8. DOI: 10.1159/000182649. View

11.

Lin C . Treatment of hepatitis B virus-associated membranous nephropathy with recombinant alpha-interferon. Kidney Int. 1995; 47(1):225-30. DOI: 10.1038/ki.1995.27. View

12.

He X, Fang L, Zhang Y, Sheng F, Zhang X, Guo M . In situ hybridization of hepatitis B DNA in hepatitis B-associated glomerulonephritis. Pediatr Nephrol. 1998; 12(2):117-20. DOI: 10.1007/s004670050417. View

13.

Li L, Li L, Chen H, Zhou H, Ji D, Tang Z . Primary glomerulonephritis in China. Analysis of 1001 cases. Chin Med J (Engl). 1989; 102(3):159-64. View

14.

Nagy J, BAJTAI G, Brasch H, Sule T, Ambrus M, Deak G . The role of hepatitis B surface antigen in the pathogenesis of glomerulopathies. Clin Nephrol. 1979; 12(3):109-16. View

15.

Takeda S, Kida H, Katagiri M, Yokoyama H, Abe T, Hattori N . Characteristics of glomerular lesions in hepatitis B virus infection. Am J Kidney Dis. 1988; 11(1):57-62. DOI: 10.1016/s0272-6386(88)80176-8. View

16.

Knieser M, Jenis E, Lowenthal D, Bancroft W, Burns W, SHALHOUB R . Pathogenesis of renal disease associated with viral hepatitis. Arch Pathol. 1974; 97(4):193-200. View

17.

Ishihara T, AKAMATSU A, Takahashi M, Yamashita Y, Yokota T, Nagasawa T . Ultrastructure of kidney from three patients with HBeAg-associated nephropathy with special reference to virus-like particles in the glomerular tufts. Acta Pathol Jpn. 1988; 38(3):339-50. DOI: 10.1111/j.1440-1827.1988.tb02306.x. View

18.

Zhou W, Zhang W, Geng L . [Glomerulonephropathy associated with hepatitis B virus (HBV) infection]. Zhonghua Nei Ke Za Zhi. 1990; 29(9):530-3, 574. View

19.

Johnson R, Couser W . Hepatitis B infection and renal disease: clinical, immunopathogenetic and therapeutic considerations. Kidney Int. 1990; 37(2):663-76. DOI: 10.1038/ki.1990.32. View

20.

Farza H, Hadchouel M, Scotto J, Tiollais P, Babinet C, Pourcel C . Replication and gene expression of hepatitis B virus in a transgenic mouse that contains the complete viral genome. J Virol. 1988; 62(11):4144-52. PMC: 253846. DOI: 10.1128/JVI.62.11.4144-4152.1988. View