Silencing Near TRNA Genes Requires Nucleolar Localization

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2005 Jan 18

PMID 15654076

Citations 43

Authors

Li Wang

Rebecca A Haeusler

Paul D Good

Martin Thompson

Sapna Nagar

David R Engelke

Affiliations

Soon will be listed here.

Abstract

Transcription by RNA polymerase II is antagonized by the presence of a nearby tRNA gene in Saccharomyces cerevisiae. To test hypotheses concerning the mechanism of this tRNA gene-mediated (tgm) silencing, the effects of specific gene deletions were determined. The results show that the mechanism of silencing near tRNA genes is fundamentally different from other forms of transcriptional silencing in yeast. Rather, tgm silencing is dependent on the ability to cluster the dispersed tRNA genes in or near the nucleolus, constituting a form of three-dimensional gene control.

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